Literature DB >> 24597567

PEGylation of αα-Hb using succinimidyl propionic acid PEG 5K: Conjugation chemistry and PEG shell structure dictate respectively the oxygen affinity and resuscitation fluid like properties of PEG αα-Hbs.

Fantao Meng1, Amy G Tsai, Marcos Intaglietta, Seetharama A Acharya.   

Abstract

PEGylation of intramolecularly crosslinked Hb has been studied here to overcome the limitation of dissociation of Hb tetramers. New hexa and deca PEGylated low oxygen affinity PEG-ααHbs have been generated. Influence of PEG conjugation chemistry and the PEG shell structure on the functional properties as well as PEGylation induced plasma expander like properties of the protein has been delineated. The results have established that in the design of PEG-Hbs as oxygen therapeutics, the influence of conjugation chemistry and the PEG shell structure on the oxygen affinity of Hb needs to be optimized independently besides optimizing the PEG shell structure for inducing resuscitation fluid like properties.

Entities:  

Keywords:  PEG shell; PEGylated hemoglobin; PEGylation; blood substitute; oxygen affinity; plasma expander

Mesh：

Substances：

Year:  2014        PMID： 24597567      PMCID： PMC4167218          DOI： 10.3109/21691401.2014.885443

Source DB:  PubMed          Journal:  Artif Cells Nanomed Biotechnol        ISSN： 2169-1401            Impact factor:   5.678


  40 in total

1.  Influence of the chemistry of conjugation of poly(ethylene glycol) to Hb on the oxygen-binding and solution properties of the PEG-Hb conjugate.

Authors:  Tao Hu; Muthuchidambaram Prabhakaran; Seetharama A Acharya; Belur N Manjula
Journal:  Biochem J       Date:  2005-12-15       Impact factor: 3.857

2.  Enhanced molecular volume of conservatively pegylated Hb: (SP-PEG5K)6-HbA is non-hypertensive.

Authors:  Seetharama A Acharya; Joel M Friedman; Belur N Manjula; Marcos Intaglietta; Amy G Tsai; Robert M Winslow; Ashok Malavalli; Kim Vandegriff; Paul K Smith
Journal:  Artif Cells Blood Substit Immobil Biotechnol       Date:  2005

3.  Extension arm facilitated PEGylation of hemoglobin: correlation of the properties with the extent of PEGylation.

Authors:  Dongxia Li; Belur N Manjula; A Seetharama Acharya
Journal:  Protein J       Date:  2006-06       Impact factor: 2.371

4.  Non-conservative surface decoration of hemoglobin: influence of neutralization of positive charges at PEGylation sites on molecular and functional properties of PEGylated hemoglobin.

Authors:  Dongxia Li; Tao Hu; Belur N Manjula; Seetharama A Acharya
Journal:  Biochim Biophys Acta       Date:  2008-04-11

5.  Oxidation and haem loss kinetics of poly(ethylene glycol)-conjugated haemoglobin (MP4): dissociation between in vitro and in vivo oxidation rates.

Authors:  Kim D Vandegriff; Ashok Malavalli; Charles Minn; Eva Jiang; Jeff Lohman; Mark A Young; Michele Samaja; Robert M Winslow
Journal:  Biochem J       Date:  2006-11-01       Impact factor: 3.857

6.  Influence of intramolecular cross-links on the molecular, structural and functional properties of PEGylated haemoglobin.

Authors:  Tao Hu; Belur N Manjula; Dongxia Li; Michael Brenowitz; Seetharama A Acharya
Journal:  Biochem J       Date:  2007-02-15       Impact factor: 3.857

7.  Combining the influence of two low O2 affinity-inducing chemical modifications of the central cavity of hemoglobin.

Authors:  Parimala Nacharaju; Joel M Friedman; Muthuchidambaram Prabhakaran; Seetharama A Acharya; Belur N Manjula
Journal:  Biochemistry       Date:  2007-03-24       Impact factor: 3.162

8.  Volume resuscitation from hemorrhagic shock with albumin and hexaPEGylated human serum albumin.

Authors:  Pedro Cabrales; Amy G Tsai; K Ananda; Seetharama A Acharya; Marcos Intaglietta
Journal:  Resuscitation       Date:  2008-07-14       Impact factor: 5.262

9.  Non-hypertensive tetraPEGylated canine haemoglobin: correlation between PEGylation, O2 affinity and tissue oxygenation.

Authors:  Seetharama A Acharya; Vivek N Acharya; Nirmala Devi Kanika; Amy G Tsai; Marcos Intaglietta; Belur N Manjula
Journal:  Biochem J       Date:  2007-08-01       Impact factor: 3.857

Review 10.  Is the distribution of tissue pO(2) homogeneous?

Authors:  Amy G Tsai; Paul C Johnson; Marcos Intaglietta
Journal:  Antioxid Redox Signal       Date:  2007-07       Impact factor: 8.401
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  1 in total

1.  Numerical investigation of oxygen transport by hemoglobin-based carriers through microvessels.

Authors:  Toru Hyakutake; Takumi Kishimoto
Journal:  J Artif Organs       Date:  2017-07-28       Impact factor: 1.731
  1 in total

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