S Hong1, B V Alapure1, Y Lu1, H Tian1, Q Wang1. 1. Center of Neuroscience Excellence, Louisiana State University Health Science Center, New Orleans, LA 70112.
Abstract
BACKGROUND: Mesenchymal stem cells (MSCs) promote skin healing. 12/15-Lipoxgenase (LOX) is crucial in producing specific lipid mediators in wounded skin. The consequences of 12/15-LOX deficiency in MSC densities in skin are unknown. OBJECTIVES: To determine the effect of 12/15-LOX deficiency in MSC densities in wounded and unwounded dermis. METHODS: Full-thickness skin incisional wounds were made to 12/15-LOX-deficient (12/15-LOX(-/-) ) and wild-type (WT) C57BL/6 mice. Wounded skin was collected at 3, 8, or 14 days postwounding (dpw). MSCs were analysed in skin sections using histology. 12S- or 15S-hydroxy-eicosatetraenoic acid (HETE) was analysed using a reversed-phase Chiral liquid chromatography-ultraviolet-tandem mass spectrometer. RESULTS: There were more stem cell antigen (Sca)1(+) CD29(+) MSCs (cells/field) at 3, 8, and 14 dpw, more Sca1(+) CD106(+) MSCs at 3 and 14 dpw in the wounded dermis, more MSCs in unwounded dermis of WT mice compared with 12/15-LOX(-/-) mice, and more MSCs in the wounded dermis than in the unwounded dermis. For 12/15-LOX(-/-) dermis, Sca1(+) CD106(+) MSCs peaked and Sca1(+) CD29(+) MSCs reached a flat level at 8 dpw. However, for the WT dermis, MSCs increased from 8 to 14 dpw. There were more Sca1(+) CD106(+) MSCs than Sca1(+) CD29(+) MSCs in the 12/15-LOX(-/-) wounded dermis at 8 dpw. However, there were more Sca1(+) CD29(+) MSCs in the 12/15-LOX(-/-) than Sca1(+) CD106(+) MSCs in the WT wounded dermis at 3 dpw, and Sca1(+) CD106(+) MSCs and Sca1(+) CD29(+) MSCs were at comparable levels in other conditions. 12/15-LOX deficiency suppressed levels of 12/15-LOX protein and their products, 12S-HETE and 15S-HETE, in wounds. CONCLUSIONS: 12/15-LOX deficiency reduces MSC densities in the dermis, which correlates with the suppressed 12/15-LOX pathways in wounded and unwounded skin.
BACKGROUND: Mesenchymal stem cells (MSCs) promote skin healing. 12/15-Lipoxgenase (LOX) is crucial in producing specific lipid mediators in wounded skin. The consequences of 12/15-LOX deficiency in MSC densities in skin are unknown. OBJECTIVES: To determine the effect of 12/15-LOX deficiency in MSC densities in wounded and unwounded dermis. METHODS: Full-thickness skin incisional wounds were made to 12/15-LOX-deficient (12/15-LOX(-/-) ) and wild-type (WT) C57BL/6 mice. Wounded skin was collected at 3, 8, or 14 days postwounding (dpw). MSCs were analysed in skin sections using histology. 12S- or 15S-hydroxy-eicosatetraenoic acid (HETE) was analysed using a reversed-phase Chiral liquid chromatography-ultraviolet-tandem mass spectrometer. RESULTS: There were more stem cell antigen (Sca)1(+) CD29(+) MSCs (cells/field) at 3, 8, and 14 dpw, more Sca1(+) CD106(+) MSCs at 3 and 14 dpw in the wounded dermis, more MSCs in unwounded dermis of WT mice compared with 12/15-LOX(-/-) mice, and more MSCs in the wounded dermis than in the unwounded dermis. For 12/15-LOX(-/-) dermis, Sca1(+) CD106(+) MSCs peaked and Sca1(+) CD29(+) MSCs reached a flat level at 8 dpw. However, for the WT dermis, MSCs increased from 8 to 14 dpw. There were more Sca1(+) CD106(+) MSCs than Sca1(+) CD29(+) MSCs in the 12/15-LOX(-/-) wounded dermis at 8 dpw. However, there were more Sca1(+) CD29(+) MSCs in the 12/15-LOX(-/-) than Sca1(+) CD106(+) MSCs in the WT wounded dermis at 3 dpw, and Sca1(+) CD106(+) MSCs and Sca1(+) CD29(+) MSCs were at comparable levels in other conditions. 12/15-LOX deficiency suppressed levels of 12/15-LOX protein and their products, 12S-HETE and 15S-HETE, in wounds. CONCLUSIONS: 12/15-LOX deficiency reduces MSC densities in the dermis, which correlates with the suppressed 12/15-LOX pathways in wounded and unwounded skin.
Authors: Oren M Tepper; Jacquelyn Carr; Robert J Allen; Christopher C Chang; Clarence D Lin; Rica Tanaka; Sanjeev M Gupta; Jamie P Levine; Pierre B Saadeh; Stephen M Warren Journal: Diabetes Date: 2010-05-18 Impact factor: 9.461
Authors: Jorgelina M Calandria; Victor L Marcheselli; Pranab K Mukherjee; Jasim Uddin; Jeremy W Winkler; Nicos A Petasis; Nicolas G Bazan Journal: J Biol Chem Date: 2009-04-29 Impact factor: 5.157
Authors: Bhagwat V Alapure; Yan Lu; Mingyu He; Chih-Chang Chu; Hongying Peng; Filipe Muhale; Yue-Liang Brewerton; Bruce Bunnell; Song Hong Journal: Stem Cells Dev Date: 2018-10-23 Impact factor: 3.272
Authors: Jason Hellmann; Brian E Sansbury; Blenda Wong; Xiaofeng Li; Mansher Singh; Kristo Nuutila; Nan Chiang; Elof Eriksson; Charles N Serhan; Matthew Spite Journal: J Invest Dermatol Date: 2018-03-17 Impact factor: 8.551
Authors: Hernan A Bazan; Yan Lu; Bokkyoo Jun; Zhide Fang; T Cooper Woods; Song Hong Journal: Prostaglandins Leukot Essent Fatty Acids Date: 2017-08-26 Impact factor: 4.006
Authors: Song Hong; Aarti Nagayach; Yan Lu; Hongying Peng; Quoc-Viet A Duong; Nicholas B Pham; Christopher A Vuong; Nicolas G Bazan Journal: CNS Neurosci Ther Date: 2021-09-12 Impact factor: 5.243