Serap Cilaker Mıcılı1, Aslı Göker2, Oya Sayın3, Pınar Akokay1, Bekir Uğur Ergür1. 1. Department of Histology and Embryology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey. 2. Department of Obstetrics and Gynecology, Celal Bayar University Faculty of Medicine, Manisa, Turkey. 3. Department of Biochemistry, Dokuz Eylül University Learning Resources Center, İzmir, Turkey.
Abstract
OBJECTIVE: To investigate the effects of lipoic acid in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemistry in a rat uterine horn model with full thickness injury. MATERIAL AND METHODS: Twenty-eight female Wistar albino rats were randomised into four groups: uterine trauma control, 15 days and 30 days, and uterine trauma + lipoic acid, 15 days and 30 days. A full thickness defect was established by incising a segment of approximately 1.0 cm in length from each uterine horn, leaving the mesometrium intact. Extension and severity of the adhesions in each group were scored by a visual scoring system and evaluated immunohistochemically. RESULTS: Adhesion scores were 2.00±0.81, 2.14±0.69 0.71±0.75, and 0.85±0.69 for extent and 2.28±0.48, 2.14±0.69, 0.85±0.69, and 1.14±0.69 for severity in Groups 1, 2, 3 and 4, respectively. Adhesion extent and severity were significantly less for groups treated by lipoic acid but no difference was observed between long and short administration. Both Vitronectin and u-PAR staining were significantly increased in treatment groups when compared to the control group. CONCLUSION: Lipoic acid was found to be effective in reducing postoperative adhesion formation in a rat model.
OBJECTIVE: To investigate the effects of lipoic acid in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemistry in a rat uterine horn model with full thickness injury. MATERIAL AND METHODS: Twenty-eight female Wistar albino rats were randomised into four groups: uterine trauma control, 15 days and 30 days, and uterine trauma + lipoic acid, 15 days and 30 days. A full thickness defect was established by incising a segment of approximately 1.0 cm in length from each uterine horn, leaving the mesometrium intact. Extension and severity of the adhesions in each group were scored by a visual scoring system and evaluated immunohistochemically. RESULTS: Adhesion scores were 2.00±0.81, 2.14±0.69 0.71±0.75, and 0.85±0.69 for extent and 2.28±0.48, 2.14±0.69, 0.85±0.69, and 1.14±0.69 for severity in Groups 1, 2, 3 and 4, respectively. Adhesion extent and severity were significantly less for groups treated by lipoic acid but no difference was observed between long and short administration. Both Vitronectin and u-PAR staining were significantly increased in treatment groups when compared to the control group. CONCLUSION:Lipoic acid was found to be effective in reducing postoperative adhesion formation in a rat model.
Authors: Arin K Greene; Ian P J Alwayn; Vania Nose; Evelyn Flynn; David Sampson; David Zurakowski; Judah Folkman; Mark Puder Journal: Ann Surg Date: 2005-07 Impact factor: 12.969
Authors: Sander ten Raa; M Petrousjka van den Tol; Wim Sluiter; Leo J Hofland; Casper H J van Eijck; Hans Jeekel Journal: J Surg Res Date: 2006-09-27 Impact factor: 2.192