Gazi Yıldırım1, Rukset Attar1, Cem Fıçıcıoğlu2, Ateş Karateke1, Ferda Ozkan3, Ertuğrul Kılıç4, Bayram Yılmaz5, Narter Yeşildağlar1. 1. Yeditepe University Hospital, Department of Obstetrics, and Gynecology, Istanbul, Turkey. 2. Yeditepe University Hospital, Center For Reproductive Medicine, Head of The Department of Obstetrics, and Gynecology, Istanbul, Turkey. 3. Yeditepe University Hospital, Department of Pathology, Istanbul, Turkey. 4. Yeditepe University Hospital, Department of Physiology, Istanbul, Turkey. 5. Yeditepe University Hospital, Head of The Department of Physiology and The Director of Yeditepe University Experimental Research Center (YUDETAM).
Abstract
OBJECTIVE: To determine the effects of the combination of letrozole and melatonin on surgically induced endometriosis. MATERIAL AND METHODS: This prospective, randomized, controlled, experimental study was carried out at Yeditepe University Experimental Research Center (YUDETAM). Female non-pregnant, 17 nulligravid Wistar - Hannover albino rats with surgically induced endometriosis were used in this study. Endometriosis was induced by using homologous uterine horn transplantation in the rats. Four operations were performed on each rat. The induction of endometriosis was performed in the first operation. After two weeks of estradiol treatment the second operation was performed and endometriotic lesions were evaluated. Estrogen was then discontinued and in the study groups medications were started. During two weeks the rats were given medications and the third operation was performed for the assessment of the effects of the medications on the endometriotic foci. Then all the medications were stopped and estrogen was started again. Two weeks later all the rats were euthanized and recurrence of endometriosis was evaluated. RESULTS: The sum of the lesion volumes in the control group was 93.6±31.7 mm(3) at the end of the second week. After the cessation of estradiol it decreased to 85.0±23.8 mm(3) (P=0.31) and increased to 119.7±29.4 mm(3) at the sixth week (P=0.02). A significant reduction in histopathologic scores were seen after cessation of the estradiol (p=0.04). At the end of the sixth week, histopathological scores reached the pretreatment values. In the letrozole and melatonin group the sum of the lesion volumes decreased significantly after the treatment (82.8±21.0 mm(3) and 15.7±8.0 mm(3) respectively). At the end of the sixth week, the mean volume was calculated as 43.9±31.8 mm(3) (p=0.002). Histopathologic scores were 2.3±0.1, 2.0±0.2 and 2.2±0.3 at the end of the second, fourth and sixth weeks, respectively, in the letrozole and melatonin group. CONCLUSIONS: Letrozole and melatonin caused a significant regression in lesion volumes; however, histopathological scores of endometriotic lesions did not change significantly.
OBJECTIVE: To determine the effects of the combination of letrozole and melatonin on surgically induced endometriosis. MATERIAL AND METHODS: This prospective, randomized, controlled, experimental study was carried out at Yeditepe University Experimental Research Center (YUDETAM). Female non-pregnant, 17 nulligravid Wistar - Hannover albino rats with surgically induced endometriosis were used in this study. Endometriosis was induced by using homologous uterine horn transplantation in the rats. Four operations were performed on each rat. The induction of endometriosis was performed in the first operation. After two weeks of estradiol treatment the second operation was performed and endometriotic lesions were evaluated. Estrogen was then discontinued and in the study groups medications were started. During two weeks the rats were given medications and the third operation was performed for the assessment of the effects of the medications on the endometriotic foci. Then all the medications were stopped and estrogen was started again. Two weeks later all the rats were euthanized and recurrence of endometriosis was evaluated. RESULTS: The sum of the lesion volumes in the control group was 93.6±31.7 mm(3) at the end of the second week. After the cessation of estradiol it decreased to 85.0±23.8 mm(3) (P=0.31) and increased to 119.7±29.4 mm(3) at the sixth week (P=0.02). A significant reduction in histopathologic scores were seen after cessation of the estradiol (p=0.04). At the end of the sixth week, histopathological scores reached the pretreatment values. In the letrozole and melatonin group the sum of the lesion volumes decreased significantly after the treatment (82.8±21.0 mm(3) and 15.7±8.0 mm(3) respectively). At the end of the sixth week, the mean volume was calculated as 43.9±31.8 mm(3) (p=0.002). Histopathologic scores were 2.3±0.1, 2.0±0.2 and 2.2±0.3 at the end of the second, fourth and sixth weeks, respectively, in the letrozole and melatonin group. CONCLUSIONS:Letrozole and melatonin caused a significant regression in lesion volumes; however, histopathological scores of endometriotic lesions did not change significantly.
Entities:
Keywords:
Endometriosis; letrozole; melatonin; rat endometriosis
Authors: Lisa L Amsterdam; William Gentry; Smeta Jobanputra; Michael Wolf; Stephen D Rubin; Serdar E Bulun Journal: Fertil Steril Date: 2005-08 Impact factor: 7.329