Literature DB >> 24591688

Producing and concentrating lenti-Cre for mouse infections.

Jessica J Gierut1, Tyler E Jacks, Kevin M Haigis.   

Abstract

Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.

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Year:  2014        PMID: 24591688      PMCID: PMC4169239          DOI: 10.1101/pdb.prot073437

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  3 in total

1.  In vivo delivery of lenti-Cre or adeno-Cre into mice using intranasal instillation.

Authors:  Jessica J Gierut; Tyler E Jacks; Kevin M Haigis
Journal:  Cold Spring Harb Protoc       Date:  2014-03-01

Review 2.  Strategies to achieve conditional gene mutation in mice.

Authors:  Jessica J Gierut; Tyler E Jacks; Kevin M Haigis
Journal:  Cold Spring Harb Protoc       Date:  2014-04-01

3.  H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression.

Authors:  S P Rowbotham; F Li; A F M Dost; S M Louie; B P Marsh; P Pessina; C R Anbarasu; C F Brainson; S J Tuminello; A Lieberman; S Ryeom; T M Schlaeger; B J Aronow; H Watanabe; K K Wong; C F Kim
Journal:  Nat Commun       Date:  2018-11-19       Impact factor: 14.919

  3 in total

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