| Literature DB >> 2458521 |
Abstract
A method is presented to determine the RNA content in isolated chromatin. An increased ellipticity at 260 nm-290 nm in the circular dichroism spectrum of chromatin is due to RNA released together with chromatin from the cell nuclei. Mixtures of chicken erythrocyte chromatin and pure 5s-RNA were used to reconstruct such spectra. From the obtained data an RNA content higher than 3-5% in samples of isolated chromatin can be determined.Entities:
Mesh：
Substances：
Year: 1988 PMID： 2458521 DOI： 10.1007/bf00805634
Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316