PURPOSE: To examine the relationship between the density of tumor-infiltrating T cell subpopulations and the pathological response to induction chemoradiotherapy (CRT) in patients with locally advanced NSCLC, and to assess the impact of T cell density on patient prognosis. METHODS: A total of 64 patients with c-stages IIA-IIIB NSCLC who underwent induction CRT followed by R0 surgery were enrolled. Tumor-infiltrating T cells expressing either FOXP3 or CD8 were detected by immunohistochemical staining. RESULTS: Mean numbers of tumor-infiltrating FOXP3+ T cells were 39.9 for patients with minor pathological responses (n = 9), 18.4 for those with major pathological responses (n = 25), and 12.9 for those with complete pathological responses (n = 30; P <0.001). The number of CD8+ T cells was not associated with pathological responses. Patients with lower FOXP3+ T cell densities showed better survival, although the difference was not statistically significant. CONCLUSION: Our study demonstrated that the density of tumor-infiltrating FOXP3+ T cells indicated the degree of response for induction CRT and prognosis in patients treated with trimodality therapy for locally advanced NSCLC, suggesting that FOXP3+ T cells may be target for adjunct immunotherapy.
PURPOSE: To examine the relationship between the density of tumor-infiltrating T cell subpopulations and the pathological response to induction chemoradiotherapy (CRT) in patients with locally advanced NSCLC, and to assess the impact of T cell density on patient prognosis. METHODS: A total of 64 patients with c-stages IIA-IIIB NSCLC who underwent induction CRT followed by R0 surgery were enrolled. Tumor-infiltrating T cells expressing either FOXP3 or CD8 were detected by immunohistochemical staining. RESULTS: Mean numbers of tumor-infiltrating FOXP3+ T cells were 39.9 for patients with minor pathological responses (n = 9), 18.4 for those with major pathological responses (n = 25), and 12.9 for those with complete pathological responses (n = 30; P <0.001). The number of CD8+ T cells was not associated with pathological responses. Patients with lower FOXP3+ T cell densities showed better survival, although the difference was not statistically significant. CONCLUSION: Our study demonstrated that the density of tumor-infiltrating FOXP3+ T cells indicated the degree of response for induction CRT and prognosis in patients treated with trimodality therapy for locally advanced NSCLC, suggesting that FOXP3+ T cells may be target for adjunct immunotherapy.
Authors: Hans Van Hulle; Vincent Vakaet; Giselle Post; Annick Van Greveling; Chris Monten; An Hendrix; Koen Van de Vijver; Jo Van Dorpe; Pieter De Visschere; Geert Braems; Katrien Vandecasteele; Hannelore Denys; Wilfried De Neve; Liv Veldeman Journal: Pilot Feasibility Stud Date: 2020-10-10
Authors: Malaka Ameratunga; Khashayar Asadi; Xihui Lin; Marzena Walkiewicz; Carmel Murone; Simon Knight; Paul Mitchell; Paul Boutros; Thomas John Journal: PLoS One Date: 2016-04-22 Impact factor: 3.240
Authors: Shucai Yang; Yi Liu; Ming-Yue Li; Calvin S H Ng; Sheng-Li Yang; Shanshan Wang; Chang Zou; Yujuan Dong; Jing Du; Xiang Long; Li-Zhong Liu; Innes Y P Wan; Tony Mok; Malcolm J Underwood; George G Chen Journal: Mol Cancer Date: 2017-07-17 Impact factor: 27.401