Literature DB >> 2458368

Coumarin-phalloidin: a new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton.

J V Small1, S Zobeley, G Rinnerthaler, H Faulstich.   

Abstract

7-Diethylamino-3-(4-isothiocyanotophenyl)-4-methylcoumarin (CPITC) was coupled to amino-methyldithiolanophalloidin to produce a new phalloidin derivative, coumarin-phalloidin, fluorescent in the blue region of the spectrum. Coumarin-phalloidin binds to actin with around 100-fold less affinity than unconjugated phalloidin, but with enough avidity to make it a useful stain for actin filaments. Appropriate filter combinations permit triple immunofluorescence microscopy of the cytoskeleton with fluorescein and rhodamine conjugates together with coumarin-phalloidin.

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Year:  1988        PMID： 2458368     DOI： 10.1242/jcs.89.1.21

Source DB:  PubMed          Journal:  J Cell Sci        ISSN： 0021-9533            Impact factor:   5.285


  6 in total

1.  Non-aqueous permanent mounting for immunofluorescence microscopy.

Authors:  Jesús Espada; Angeles Juarranz; Sergio Galaz; Magdalena Cañete; Angeles Villanueva; María Pacheco; Juan C Stockert
Journal:  Histochem Cell Biol       Date:  2005-03-15       Impact factor: 4.304

2.  Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes.

Authors:  Guiting Lin; Xuefeng Qiu; Thomas M Fandel; Maarten Albersen; Zhong Wang; Tom F Lue; Ching-Shwun Lin
Journal:  Urology       Date:  2011-08-16       Impact factor: 2.649

Review 3.  Fluorescent phallotoxins as probes for filamentous actin.

Authors:  H Faulstich; S Zobeley; G Rinnerthaler; J V Small
Journal:  J Muscle Res Cell Motil       Date:  1988-10       Impact factor: 2.698

4.  Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.

Authors:  K R Loeb; A L Haas
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

5.  Keratin incorporation into intermediate filament networks is a rapid process.

Authors:  R K Miller; K Vikstrom; R D Goldman
Journal:  J Cell Biol       Date:  1991-05       Impact factor: 10.539

6.  Thrombospondin-1 Modulates Actin Filament Remodeling and Cell Motility in Mouse Mammary Tumor cells in Vitro.

Authors:  Dorothy Ndishabandi; Cameron Duquette; Ghita El-Moatassim Billah; Millys Reyes; Mark Duquette; Jack Lawler; Shideh Kazerounian
Journal:  Discoveries (Craiova)       Date:  2014 Oct-Dec
  6 in total

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