Literature DB >> 2458317

FMLP-induced arachidonic acid release, phospholipid metabolism, and calcium mobilization in human monocytes. Regulation by cyclic AMP.

R W Godfrey1, R M Manzi, B D Jensen, S T Hoffstein.   

Abstract

Radiolabeled human peripheral blood monocytes released [3H]arachidonic acid upon challenge with the calcium ionophore A23187 (10 microM), or f-Met-Leu-Phe (FMLP, 1 microM). Chromatographic analysis of [3H]arachidonic acid labeled phospholipids showed that stimulation by FMLP reduced the amount of labeled phosphatidylcholine exclusively. Treatment of the monocytes with 10(-3) M dibutyryl cyclic AMP (d-cAMP) or 5 X 10(-4) M isobutylmethylxanthine (IBMX) substantially inhibited [3H]arachidonic acid release (30%) and depletion from labeled phosphatidylcholine (PC) in FMLP--but not calcium ionophore--stimulated cells. Using the fluorescent probe Indo-1, the FMLP-induced cytosolic calcium increase was unaffected by 10(-3) M dibutyryl cyclic AMP. The results suggest that FMLP-stimulated phospholipase activity is regulated by cyclic AMP, but not by depressing receptor-medicated increases in cytoplasmic free calcium.

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Year:  1988        PMID： 2458317     DOI： 10.1007/bf00920074

Source DB:  PubMed          Journal:  Inflammation        ISSN： 0360-3997            Impact factor:   4.092


  16 in total

1.  The activation by Ca2+ of platelet phospholipase A2. Effects of dibutyryl cyclic adenosine monophosphate and 8-(N,N-diethylamino)-octyl-3,4,5-trimethoxybenzoate.

Authors:  S Rittenhouse-Simmons; D Deykin
Journal:  Biochim Biophys Acta       Date:  1978-11-01

2.  Rapid killing of actinomycin D-treated tumor cells by human mononuclear cells. I. Effectors belong to the monocyte-macrophage lineage.

Authors:  F Colotta; G Peri; A Villa; A Mantovani
Journal:  J Immunol       Date:  1984-02       Impact factor: 5.422

3.  Binding of protein kinase substrates by fluorescently labeled calmodulin.

Authors:  D A Malencik; T S Huang; S R Anderson
Journal:  Biochem Biophys Res Commun       Date:  1982-09-16       Impact factor: 3.575

4.  Inhibitory action of cyclic GMP on secretion, polyphosphoinositide hydrolysis and calcium mobilization in thrombin-stimulated human platelets.

Authors:  S Nakashima; T Tohmatsu; H Hattori; Y Okano; Y Nozawa
Journal:  Biochem Biophys Res Commun       Date:  1986-03-28       Impact factor: 3.575

5.  Regulation of the intracellular calcium level in human blood platelets: cyclic adenosine 3',5'-monophosphate dependent phosphorylation of a 22,000 dalton component in isolated Ca2+-accumulating vesicles.

Authors:  R Käser-Glanzmann; E Gerber; E F Lüscher
Journal:  Biochim Biophys Acta       Date:  1979-12-12

6.  Physiological and pharmacological regulation of prostaglandin and leukotriene production by macrophages.

Authors:  R J Bonney; J L Humes
Journal:  J Leukoc Biol       Date:  1984-01       Impact factor: 4.962

Review 7.  Inositol trisphosphate, a novel second messenger in cellular signal transduction.

Authors:  M J Berridge; R F Irvine
Journal:  Nature       Date:  1984 Nov 22-28       Impact factor: 49.962

8.  Substrate-specific forms of human platelet phospholipase A2.

Authors:  L R Ballou; L M DeWitt; W Y Cheung
Journal:  J Biol Chem       Date:  1986-03-05       Impact factor: 5.157

9.  Phospholipid and neutral lipid separation by one-dimensional thin-layer chromatography.

Authors:  K Korte; M L Casey
Journal:  J Chromatogr       Date:  1982-10-08

10.  Quantitative analysis of the cytosolic free calcium dependency of exocytosis from three subcellular compartments in intact human neutrophils.

Authors:  P D Lew; A Monod; F A Waldvogel; B Dewald; M Baggiolini; T Pozzan
Journal:  J Cell Biol       Date:  1986-06       Impact factor: 10.539
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