OBJECTIVE: To evaluate if 14 genes that discriminate metastasising and non-metastasising human uveal melanomas can differentiate metastasising and non-metastasising uveal melanomas in dogs. METHODS: Nineteen archival biopsies of eyes with a histopathological classification of primary benign (n = 9) and malignant (n = 10) uveal melanoma were selected. Thoracic and/or abdominal metastases confirmed metastatic spread of the primary tumour in seven dogs during the follow-up period. Gene expression was assayed by Reverse Transcription-quantitative Polymerase Chain Reaction. Genes displaying statistically significant differences in expression between the metastasising and non-metastasising tumours were identified. RESULTS: Four genes (HTR2B, FXR1, LTA4H and CDH1) demonstrated increased expression in the metastasising uveal melanomas. CLINICAL SIGNIFICANCE: This preliminary study illustrates the potential utility of gene expression markers for predicting canine uveal melanoma metastasis. The genes displaying elevated expression in the metastasising tumours are part of a 12-discriminating gene set used in a routine assay, performed on fine needle aspirate biopsies collected without enucleation, for predicting human uveal melanoma metastasis. Further work is required to validate the results.
OBJECTIVE: To evaluate if 14 genes that discriminate metastasising and non-metastasising humanuveal melanomas can differentiate metastasising and non-metastasising uveal melanomas in dogs. METHODS: Nineteen archival biopsies of eyes with a histopathological classification of primary benign (n = 9) and malignant (n = 10) uveal melanoma were selected. Thoracic and/or abdominal metastases confirmed metastatic spread of the primary tumour in seven dogs during the follow-up period. Gene expression was assayed by Reverse Transcription-quantitative Polymerase Chain Reaction. Genes displaying statistically significant differences in expression between the metastasising and non-metastasising tumours were identified. RESULTS: Four genes (HTR2B, FXR1, LTA4H and CDH1) demonstrated increased expression in the metastasising uveal melanomas. CLINICAL SIGNIFICANCE: This preliminary study illustrates the potential utility of gene expression markers for predicting canineuveal melanoma metastasis. The genes displaying elevated expression in the metastasising tumours are part of a 12-discriminating gene set used in a routine assay, performed on fine needle aspirate biopsies collected without enucleation, for predicting humanuveal melanoma metastasis. Further work is required to validate the results.
Authors: Laura Nordio; Chiara Bazzocchi; Francesca Genova; Valentina Serra; Maria Longeri; Giovanni Franzo; Marco Rondena; Damiano Stefanello; Chiara Giudice Journal: Front Vet Sci Date: 2021-12-13
Authors: Louise van der Weyden; Thomas Brenn; E Elizabeth Patton; Geoffrey A Wood; David J Adams Journal: J Pathol Date: 2020-07-31 Impact factor: 7.996
Authors: Tarcísio Guerra Guimarães; Karla Menezes Cardoso; Pedro Tralhão; Carlos Miguel Marto; Nuno Alexandre; Maria Filomena Botelho; Mafalda Laranjo Journal: Bioengineering (Basel) Date: 2021-12-20