| Literature DB >> 24575243 |
Darah Ibrahim1, Han Li Zhu1, Nuraqilah Yusof1, Lim Sheh Hong1.
Abstract
A total of 34 bacterial isolates were obtained from soil samples collected from Changar Hot Spring, Malang, Indonesia. Of these, 13 isolates produced a zone of hydrolysis in starch-nutrient agar medium and generated various amylases in liquid medium. One isolate was selected as the best amylase producer and was identified as Bacillus licheniformis BT5.9. The improvement of culture conditions (initial medium pH of 5.0, cultivation temperature of 50°C, agitation speed of 100 rpm and inoculum size of 1.7 × 10(9) cells/ml) provided the highest amylase production (0.327 U/ml).Entities:
Keywords: Amylase; Bacillus licheniformis; Hot Spring; Thermophilic Bacteria
Year: 2013 PMID: 24575243 PMCID: PMC3799411
Source DB: PubMed Journal: Trop Life Sci Res ISSN: 1985-3718