Literature DB >> 24573945

An improved technique for the rapid chemical characterisation of bacterial terpene cyclases.

Jeroen S Dickschat1, Khomaizon A K Pahirulzaman, Patrick Rabe, Tim A Klapschinski.   

Abstract

A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  GC/MS; actinomycetes; biosynthesis; enzymes; heterologous expression; terpene cyclases

Mesh：

Substances：

Year:  2014        PMID： 24573945     DOI： 10.1002/cbic.201300763

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  11 in total

1.  Mechanistic Insights into the Formation of the 6,10-Bicyclic Eunicellane Skeleton by the Bacterial Diterpene Synthase Bnd4.

Authors:  Baofu Xu; Dean J Tantillo; Jeffrey D Rudolf
Journal:  Angew Chem Int Ed Engl       Date:  2021-09-20       Impact factor: 16.823

2.  Knock-down of the methyltransferase Kmt6 relieves H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in Fusarium fujikuroi.

Authors:  Lena Studt; Sarah M Rösler; Immo Burkhardt; Birgit Arndt; Michael Freitag; Hans-Ulrich Humpf; Jeroen S Dickschat; Bettina Tudzynski
Journal:  Environ Microbiol       Date:  2016-07-18       Impact factor: 5.491

3.  The EIMS fragmentation mechanisms of the sesquiterpenes corvol ethers A and B, epi-cubebol and isodauc-8-en-11-ol.

Authors:  Patrick Rabe; Jeroen S Dickschat
Journal:  Beilstein J Org Chem       Date:  2016-07-05       Impact factor: 2.883

4.  Mechanistic investigations on six bacterial terpene cyclases.

Authors:  Patrick Rabe; Thomas Schmitz; Jeroen S Dickschat
Journal:  Beilstein J Org Chem       Date:  2016-08-15       Impact factor: 2.883

5.  A detailed view on 1,8-cineol biosynthesis by Streptomyces clavuligerus.

Authors:  Jan Rinkel; Patrick Rabe; Laura Zur Horst; Jeroen S Dickschat
Journal:  Beilstein J Org Chem       Date:  2016-11-04       Impact factor: 2.883

6.  The GATA-Type Transcription Factor Csm1 Regulates Conidiation and Secondary Metabolism in Fusarium fujikuroi.

Authors:  Eva-Maria Niehaus; Julia Schumacher; Immo Burkhardt; Patrick Rabe; Eduard Spitzer; Martin Münsterkötter; Ulrich Güldener; Christian M K Sieber; Jeroen S Dickschat; Bettina Tudzynski
Journal:  Front Microbiol       Date:  2017-06-26       Impact factor: 5.640

7.  Two Diterpene Synthases from Chryseobacterium: Chryseodiene Synthase and Wanjudiene Synthase.

Authors:  Lukas Lauterbach; Bernd Goldfuss; Jeroen S Dickschat
Journal:  Angew Chem Int Ed Engl       Date:  2020-05-18       Impact factor: 15.336

8.  Terpene Synthase Genes Originated from Bacteria through Horizontal Gene Transfer Contribute to Terpenoid Diversity in Fungi.

Authors:  Qidong Jia; Xinlu Chen; Tobias G Köllner; Jan Rinkel; Jianyu Fu; Jessy Labbé; Wangdan Xiong; Jeroen S Dickschat; Jonathan Gershenzon; Feng Chen
Journal:  Sci Rep       Date:  2019-06-25       Impact factor: 4.379

9.  Mechanistic Studies on Trichoacorenol Synthase from Amycolatopsis benzoatilytica.

Authors:  Jan Rinkel; Jeroen S Dickschat
Journal:  Chembiochem       Date:  2019-11-07       Impact factor: 3.164

10.  18-Hydroxydolabella-3,7-diene synthase - a diterpene synthase from Chitinophaga pinensis.

Authors:  Jeroen S Dickschat; Jan Rinkel; Patrick Rabe; Arman Beyraghdar Kashkooli; Harro J Bouwmeester
Journal:  Beilstein J Org Chem       Date:  2017-08-23       Impact factor: 2.883
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