I Nursakinah1, H A Zulkhairi2, M Norhafizah3, B Hasnah1, Md S Zamree4, Shafeera I Farrah2, D Razif2, Fansuri H Hamzah2. 1. Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 2. Department of Basic Sciences, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Selangor, Malaysia. 3. Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. 4. Herbal Technology Centre, Forest Research Institute of Malaysia, 52109 Kepong, Selangor, Malaysia.
Abstract
INTRODUCTION: The objective of this study was to determine antioxidant potential of Garcinia atroviridis leaves and fruits extracts in vitro. METHODS: Antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Total phenolic content (TPC) of the extracts was estimated as gallic acid equivalent by Folin-Ciocalteau method. Proximate analysis was determined based on the Association of Official Analytical Chemists (AOAC) procedures. RESULTS: Garcinia atroviridis leaves extracted at 100 degrees C/15 min demonstrated the highest TPC value (21.21 +/- 0.28 mg GAE/mg) and was significantly different (p < 0.05) from that of leaves extracted at 60 degrees C/6 h and 40 degrees C/12 h. On the other hand, the fruit extracted at 60 degrees C/6 h showed the highest TPC value (16.23 +/- 0.18 mg GAE/mg) (p < 0.05) compared to the fruit extracted at 40 degrees C/12 h and 100 degrees C/15 h respectively. The antioxidant activities of both samples were positively correlated with the TPC values based on DPPH-radical-scavenging activity and ferric reducing power estimation. Garcinia atroviridis leaf extract contained significantly higher proteins, carbohydrate and ash contents (2.16% +/- 0.08; 15.98% +/- 0.12 and 0.72% +/- 0.07 respectively) than its fruit extract (0.46% +/- 0.08, 8.64% +/- 0.06 and 0.15% +/- 0.06) respectively). The energy content was also found to be higher in the leaf (73.64% +/- 2.15) compared to the fruit (38.38% +/- 1.72) (p < 0.05). CONCLUSION: The findings indicate that G. atroviridis leaves and fruits have potential for use as a source of natural antioxidants and nutrients for therapeutic purposes against free radical mediated health conditions.
INTRODUCTION: The objective of this study was to determine antioxidant potential of Garcinia atroviridis leaves and fruits extracts in vitro. METHODS: Antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Total phenolic content (TPC) of the extracts was estimated as gallic acid equivalent by Folin-Ciocalteau method. Proximate analysis was determined based on the Association of Official Analytical Chemists (AOAC) procedures. RESULTS:Garcinia atroviridis leaves extracted at 100 degrees C/15 min demonstrated the highest TPC value (21.21 +/- 0.28 mg GAE/mg) and was significantly different (p < 0.05) from that of leaves extracted at 60 degrees C/6 h and 40 degrees C/12 h. On the other hand, the fruit extracted at 60 degrees C/6 h showed the highest TPC value (16.23 +/- 0.18 mg GAE/mg) (p < 0.05) compared to the fruit extracted at 40 degrees C/12 h and 100 degrees C/15 h respectively. The antioxidant activities of both samples were positively correlated with the TPC values based on DPPH-radical-scavenging activity and ferric reducing power estimation. Garcinia atroviridis leaf extract contained significantly higher proteins, carbohydrate and ash contents (2.16% +/- 0.08; 15.98% +/- 0.12 and 0.72% +/- 0.07 respectively) than its fruit extract (0.46% +/- 0.08, 8.64% +/- 0.06 and 0.15% +/- 0.06) respectively). The energy content was also found to be higher in the leaf (73.64% +/- 2.15) compared to the fruit (38.38% +/- 1.72) (p < 0.05). CONCLUSION: The findings indicate that G. atroviridis leaves and fruits have potential for use as a source of natural antioxidants and nutrients for therapeutic purposes against free radical mediated health conditions.
Authors: Wai Feng Lim; Suriati Mohd Nasir; Lay Kek Teh; Richard Johari James; Mohd Hafidz Mohd Izhar; Mohd Zaki Salleh Journal: Turk J Biol Date: 2020-12-14