| Literature DB >> 24561536 |
Saurabh Mishra1, Poonam Malhotra1, Ashutosh K Gupta1, Praveen K Singh1, Saleem Javed2, Raj Kumar3.
Abstract
In the present study, radioprotective potential of Semiquinone glucoside derivative (SQGD) isolated from radioresistant bacterium Bacillus sp. INM-1 was evaluated. γ-Radiation induced protein carbonylation, plasmid DNA damage, enzyme functional impairment, lipid peroxidation, HO radicals generation and their protection by SQGD was assessed. As a result of SQGD treatment, significant inhibition (p<0.05) in protein carbonylation was observed with BSA. SQGD treatment was found to restore supercoiled (~70±3.21%) form of irradiated plasmid DNA against γ-irradiation. SQGD protects enzymes (EcoR1 and BamH1) against radiation-induced dysfunctioning. SQGD significantly inhibited (p<0.05) lipid peroxidation in liposomes, brain and liver homogenate. Higher HO(•) radicals-averting activity of SQGD was observed in the serum and liver homogenate of C57BL/6 mice against H2O2-induced oxidative stress. In conclusion, SQGD demonstrates excellent radical-scavenging activity towards bio-macromolecules in irradiated environment and can be developed as an ideal radioprotector against radiation-induced oxidative stress in future.Entities:
Keywords: Antioxidant; Carbonylation; Lipid-peroxidation; Oxidative stress; Restriction enzyme; Toxicity
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Year: 2014 PMID： 24561536 DOI： 10.1016/j.etap.2014.01.003
Source DB: PubMed Journal: Environ Toxicol Pharmacol ISSN： 1382-6689 Impact factor: 4.860