Bi-Hua Lv1, Shi-Zhen Song, Zhibing Zhang, Yong Mei, Fang-Li Ye. 1. From the Department of Pharmacy (Mr Lv), Zhongnan Hospital of Wuhan University, Wuhan, China; School of Public Health (Drs Song, Zhang, and Mei, and Mr Ye), Wuhan University of Science and Technology, Wuhan, China; and Department of Obstetrics & Gynecology (Dr Zhang), Virginia Commonwealth University, Richmond.
Abstract
OBJECTIVE: This study analyzed the level of urinary S-phenylmercapturic acid (U-SPMA) for low benzene exposure in a group of Chinese shoe-making workers. METHODS: Urinary samples from 55 workers exposed to benzene at levels lower than 10 parts per million (ppm) were collected at postshift. U-SPMA level was determined using high-performance liquid chromatography/mass spectrography (HPLC/MS) method. RESULTS: Good linearity of U-SPMA was observed within the range from 10 to 320 μg/L (r = 0.9994). Concentration of airborne benzene ranged from 0.71 to 32.17 mg/m³, and three segments were divided with different levels of exposure (≤6.0, 6.0 to 10.0, 10 to 32.5 mg/m³), the median U-SPMA concentrations were 49.55, 102.15, and 335.69 μg/g Cr, respectively. CONCLUSION: A good linear correlation was found between U-SPMA levels and airborne benzene concentrations. The selected method could be applied for detecting other working conditions in China.
OBJECTIVE: This study analyzed the level of urinary S-phenylmercapturic acid (U-SPMA) for low benzene exposure in a group of Chinese shoe-making workers. METHODS: Urinary samples from 55 workers exposed to benzene at levels lower than 10 parts per million (ppm) were collected at postshift. U-SPMA level was determined using high-performance liquid chromatography/mass spectrography (HPLC/MS) method. RESULTS: Good linearity of U-SPMA was observed within the range from 10 to 320 μg/L (r = 0.9994). Concentration of airborne benzene ranged from 0.71 to 32.17 mg/m³, and three segments were divided with different levels of exposure (≤6.0, 6.0 to 10.0, 10 to 32.5 mg/m³), the median U-SPMA concentrations were 49.55, 102.15, and 335.69 μg/g Cr, respectively. CONCLUSION: A good linear correlation was found between U-SPMA levels and airborne benzene concentrations. The selected method could be applied for detecting other working conditions in China.
Authors: Ulrike E Rolle-Kampczyk; Jan Krumsiek; Wolfgang Otto; Stefan W Röder; Tibor Kohajda; Michael Borte; Fabian Theis; Irina Lehmann; M von Bergen Journal: Metabolomics Date: 2016-03-08 Impact factor: 4.290