Manuel Weber1, Maike Büttner-Herold2, Peter Hyckel3, Patrick Moebius4, Luitpold Distel5, Jutta Ries4, Kerstin Amann2, Friedrich W Neukam4, Falk Wehrhan4. 1. Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Germany. Electronic address: manuel.weber@uk-erlangen.de. 2. Institute of Pathology, Department of Nephropathology, University of Erlangen-Nuremberg, Germany. 3. Department of Oral and Maxillofacial Surgery, University of Jena, Germany. 4. Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Germany. 5. Department of Radiation Oncology, University of Erlangen-Nuremberg, Germany.
Abstract
BACKGROUND: In solid malignancies the influence of immunological parameters - especially of macrophages - on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The aim of this study was to correlate macrophage polarization in the epithelial and stromal compartment of oscc with histopathologic parameters. METHODS: T1 and T2 oscc samples (n = 34) were used. Automated immunohistochemical staining detected CD68, CD11c, CD163 and MRC1 positive cells. All samples were completely digitalized using whole slide imaging and the number of stained cells per area was assessed quantitatively. RESULTS: Primary tumours with lymphogenic metastasis (N+) showed a significantly (p < 0.05) increased count of CD68, CD11c, CD163 and MRC1 positive cells in the epithelial fraction compared to N0 tumours. The ratio of CD163 positive cells (M2 macrophages) to CD68 positive cells (M1 and M2 macrophages) was significantly (p < 0.05) increased in N+ tumours. CONCLUSION: An increased macrophage infiltration and an increased M2 polarization in primary oral squamous cell carcinomas with lymphogenic metastasis was shown. Macrophages that migrated into the epithelial tumour fraction seem to be of special biological importance. The results indicate a central role of macrophages in the progression of oscc.
BACKGROUND: In solid malignancies the influence of immunological parameters - especially of macrophages - on invasiveness, metastatic potential and prognosis has been shown. There are no studies quantitatively analysing the macrophage polarization in oral squamous cell carcinoma (oscc). The aim of this study was to correlate macrophage polarization in the epithelial and stromal compartment of oscc with histopathologic parameters. METHODS: T1 and T2 oscc samples (n = 34) were used. Automated immunohistochemical staining detected CD68, CD11c, CD163 and MRC1 positive cells. All samples were completely digitalized using whole slide imaging and the number of stained cells per area was assessed quantitatively. RESULTS:Primary tumours with lymphogenic metastasis (N+) showed a significantly (p < 0.05) increased count of CD68, CD11c, CD163 and MRC1 positive cells in the epithelial fraction compared to N0 tumours. The ratio of CD163 positive cells (M2 macrophages) to CD68 positive cells (M1 and M2 macrophages) was significantly (p < 0.05) increased in N+ tumours. CONCLUSION: An increased macrophage infiltration and an increased M2 polarization in primary oral squamous cell carcinomas with lymphogenic metastasis was shown. Macrophages that migrated into the epithelial tumour fraction seem to be of special biological importance. The results indicate a central role of macrophages in the progression of oscc.
Authors: Ifigeneia V Mavragani; Danae A Laskaratou; Benjamin Frey; Serge M Candéias; Udo S Gaipl; Katalin Lumniczky; Alexandros G Georgakilas Journal: Toxicol Res (Camb) Date: 2015-08-11 Impact factor: 3.524
Authors: Emma Pirilä; Otto Väyrynen; Elias Sundquist; Kaisa Päkkilä; Pia Nyberg; Sini Nurmenniemi; Virve Pääkkönen; Paula Pesonen; Dan Dayan; Marilena Vered; Lars Uhlin-Hansen; Tuula Salo Journal: PLoS One Date: 2015-03-26 Impact factor: 3.240
Authors: Falk Wehrhan; Maike Büttner-Herold; Peter Hyckel; Patrick Moebius; Raimund Preidl; Luitpold Distel; Jutta Ries; Kerstin Amann; Christian Schmitt; Friedrich W Neukam; Manuel Weber Journal: BMC Cancer Date: 2014-07-21 Impact factor: 4.430