Core-shell Ag@SiO(2) nanoparticles concentrated on a micro/nanofluidic device for surface plasmon resonance-enhanced fluorescent detection of highly reactive oxygen species.

A micro/nanofluidic device integrating a nanochannel in a microfluidic chip was developed for sensitive fluorescent determination of highly reactive oxygen species (hROS) enhanced by surface plasmon resonance-enhanced fluorescence (SPREF). The nanochannel was simply fabricated by polyaniline nanostructures modified on a glass slide. Core-shell Ag@SiO2 nanoparticles were concentrated in front of the nanochannel for fluorescence enhancement based on the SPREF effect. As a demonstration, hROS in the mainstream of cigarette smoke (CS) were detected by the present micro/nanofluidic device. The fluorescent probe for trapping hROS in puffs of CS employed a microcolumn that was loaded with a composite of DNA (conjugated fluorophores, FAM) and Au membrane (coated on cellulose acetate). With a laser-induced fluorescence detection device, hROS was determined on the basis of the amount of FAM groups generated by DNA cleavage. With the optimization of the trapping efficiency, we detected about 4.91 pmol of hROS/puff in the mainstream CS. This micro/nanofluidic-SPREF system promises a simple, rapid, and highly sensitive approach for determination of hROS in CS and other practical systems.