Literature DB >> 24554746

Comparison of a transplant multiplex viral panel on the ICEPlex system with real-time PCR for detection of cytomegalovirus, Epstein-Barr virus, and BK virus in clinical specimens.

Reeti Khare1, Mark J Espy, Jayawant N Mandrekar, Matthew J Binnicker.   

Abstract

We compared a multiplex viral transplant panel on the ICEPlex system to real-time PCR for the detection of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and BK virus (BKV). The sensitivities of the ICEPlex were 83.3%, 95.5%, and 65.5% for the detection of CMV, EBV, and BKV, respectively. Interestingly, the multiplex assay detected dual infections in 16/280 (5.7%) samples tested.

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Year:  2014        PMID： 24554746      PMCID： PMC3993682          DOI： 10.1128/JCM.00358-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

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2.  Automated high multiplex qPCR platform for simultaneous detection and quantification of multiple nucleic acid targets.

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Journal:  Biotechniques       Date:  2012-05       Impact factor: 1.993

3.  Multiplex PCR: critical parameters and step-by-step protocol.

Authors:  O Henegariu; N A Heerema; S R Dlouhy; G H Vance; P H Vogt
Journal:  Biotechniques       Date:  1997-09       Impact factor: 1.993

4.  Point: is the era of viral culture over in the clinical microbiology laboratory?

Authors:  Richard L Hodinka
Journal:  J Clin Microbiol       Date:  2012-10-10       Impact factor: 5.948

Review 5.  Multiplex polymerase chain reaction: a practical approach.

Authors:  P Markoulatos; N Siafakas; M Moncany
Journal:  J Clin Lab Anal       Date:  2002       Impact factor: 2.352
  5 in total
  5 in total

1.  Monitoring of cytomegalovirus viral loads by two molecular assays in whole-blood and plasma samples from hematopoietic stem cell transplant recipients.

Authors:  N Esther Babady; Cindy Cheng; Evelyn Cumberbatch; Jeffrey Stiles; Genovefa Papanicolaou; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2015-02-04       Impact factor: 5.948

2.  Transplant Virus Detection Using Multiplex Targeted Sequencing.

Authors:  Susanna K Tan; Peidong Shen; Martina I Lefterova; Malaya K Sahoo; Eula Fung; Justin I Odegaard; Ronald W Davis; Benjamin A Pinsky; Curt Scharfe
Journal:  J Appl Lab Med       Date:  2018-03

3.  Comparative Evaluation of Sloppy Molecular Beacon and Dual-Labeled Probe Melting Temperature Assays to Identify Mutations in Mycobacterium tuberculosis Resulting in Rifampin, Fluoroquinolone and Aminoglycoside Resistance.

Authors:  Sandy S Roh; Laura E Smith; Jong Seok Lee; Laura E Via; Clifton E Barry; David Alland; Soumitesh Chakravorty
Journal:  PLoS One       Date:  2015-05-04       Impact factor: 3.240

4.  CSF herpes virus and autoantibody profiles in the evaluation of encephalitis.

Authors:  Jenny J Linnoila; Matthew J Binnicker; Masoud Majed; Christopher J Klein; Andrew McKeon
Journal:  Neurol Neuroimmunol Neuroinflamm       Date:  2016-06-01

5.  Metagenomic Next-Generation Sequencing for Identification and Quantitation of Transplant-Related DNA Viruses.

Authors:  Meredith L Carpenter; Susanna K Tan; Thomas Watson; Rowena Bacher; Vaishnavi Nagesh; Alain Watts; Gordon Bentley; Jenna Weber; ChunHong Huang; Malaya K Sahoo; Armin Hinterwirth; Thuy Doan; Theodore Carter; Queeny Dong; Stéphane Gourguechon; Eric Harness; Sean Kermes; Srihari Radhakrishnan; Gongbo Wang; Alejandro Quiroz-Zárate; Jesus Ching; Benjamin A Pinsky
Journal:  J Clin Microbiol       Date:  2019-11-22       Impact factor: 5.948
  5 in total

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