Literature DB >> 2455457

A stain for iron-containing proteins sensitive to nanogram levels of iron.

C F Kuo1, I Fridovich.   

Abstract

A method for staining iron proteins on polyacrylamide gel electropherograms is described. It is based upon catalysis, by iron, of the oxidation of diaminobenzoic acid by hydrogen peroxide. Bands containing as little as 5 ng of protein-bound iron can be visualized by this method.

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Year:  1988        PMID： 2455457     DOI： 10.1016/0003-2697(88)90106-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Purification and characterization of NafY (apodinitrogenase gamma subunit) from Azotobacter vinelandii.

Authors:  Luis M Rubio; Steven W Singer; Paul W Ludden
Journal:  J Biol Chem       Date:  2004-03-02       Impact factor: 5.157

2.  ApoNifH functions in iron-molybdenum cofactor synthesis and apodinitrogenase maturation.

Authors:  P Rangaraj; V K Shah; P W Ludden
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-14       Impact factor: 11.205

3.  Alterations in the mucosal processing of iron in response to very-short-term dietary iron depletion and repletion.

Authors:  R W Topham; C E Eads; B F Butler
Journal:  Biochem J       Date:  1992-06-15       Impact factor: 3.857

4.  In vitro assembly of [Fe4S4] cluster in high potential iron-sulfur protein from Acidithiobacillus ferrooxidans.

Authors:  Jia Zeng; Huidan Jiang; Meimei Geng; Yiping Wang; Xiaojian Zhang; Jianshe Liu; Guanzhou Qiu
Journal:  Curr Microbiol       Date:  2008-06-24       Impact factor: 2.188

5.  Quantitative proteomics: assessing the spectrum of in-gel protein detection methods.

Authors:  Victoria J Gauci; Elise P Wright; Jens R Coorssen
Journal:  J Chem Biol       Date:  2010-06-19

6.  Genes required for rapid expression of nitrogenase activity in Azotobacter vinelandii.

Authors:  Leonardo Curatti; Carolyn S Brown; Paul W Ludden; Luis M Rubio
Journal:  Proc Natl Acad Sci U S A       Date:  2005-04-21       Impact factor: 11.205

7.  Mapping essential domains of Mycobacterium smegmatis WhmD: insights into WhiB structure and function.

Authors:  Tirumalai R Raghunand; William R Bishai
Journal:  J Bacteriol       Date:  2006-10       Impact factor: 3.490

8.  Biogenesis of iron-sulfur clusters in photosystem I: holo-NfuA from the cyanobacterium Synechococcus sp. PCC 7002 rapidly and efficiently transfers [4Fe-4S] clusters to apo-PsaC in vitro.

Authors:  Zhao Jin; Mark Heinnickel; Carsten Krebs; Gaozhong Shen; John H Golbeck; Donald A Bryant
Journal:  J Biol Chem       Date:  2008-08-11       Impact factor: 5.157

9.  The major iron-containing protein of Legionella pneumophila is an aconitase homologous with the human iron-responsive element-binding protein.

Authors:  J M Mengaud; M A Horwitz
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

10.  Perturbation of nifT expression in Klebsiella pneumoniae has limited effect on nitrogen fixation.

Authors:  H M Simon; M J Homer; G P Roberts
Journal:  J Bacteriol       Date:  1996-05       Impact factor: 3.490
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