PURPOSE: Primary vitreous regression is a critical event in mammalian eye development required for proper ocular maturity and unhindered vision. Failure of this event results in the eye disease persistent hyperplastic primary vitreous (PHPV), also identified as persistent fetal vasculature (PFV), a condition characterized by the presence of a fibrovascular mass adjacent to the lens and retina, and associated with visual disability and blindness. Here, we identify ephrin-A5 to be a critical regulator for primary vitreous regression. METHODS: Wild-type and ephrin-A5(-/-) eyes were examined at various developmental stages to determine the progression of PHPV. Eye tissue was sectioned and examined by H&E staining. Protein expression and localization was determined through immunohistochemistry. Relative levels of Eph receptors were determined by RT-PCR. RESULTS: Ephrin-A5(-/-) animals develop ocular phenotypes representative of PHPV, most notably the presence of a large hyperplastic mass posterior to the lens that remains throughout the lifetime of the animal. The aberrant tissue in these mutant mice consists of residual hyaloid vessels surrounded by pigmented cells of neural crest origin. Labeling with bromodeoxyuridine (BrdU) and detection of proliferating cell nuclear antigen (PCNA) expression shows that the mass in ephrin-A5(-/-) animals is mitotically active in embryonic and postnatal stages. CONCLUSIONS: Ephrin-A5 is a critical factor that regulates primary vitreous regression.
PURPOSE: Primary vitreous regression is a critical event in mammalian eye development required for proper ocular maturity and unhindered vision. Failure of this event results in the eye disease persistent hyperplastic primary vitreous (PHPV), also identified as persistent fetal vasculature (PFV), a condition characterized by the presence of a fibrovascular mass adjacent to the lens and retina, and associated with visual disability and blindness. Here, we identify ephrin-A5 to be a critical regulator for primary vitreous regression. METHODS: Wild-type and ephrin-A5(-/-) eyes were examined at various developmental stages to determine the progression of PHPV. Eye tissue was sectioned and examined by H&E staining. Protein expression and localization was determined through immunohistochemistry. Relative levels of Eph receptors were determined by RT-PCR. RESULTS:Ephrin-A5(-/-) animals develop ocular phenotypes representative of PHPV, most notably the presence of a large hyperplastic mass posterior to the lens that remains throughout the lifetime of the animal. The aberrant tissue in these mutant mice consists of residual hyaloid vessels surrounded by pigmented cells of neural crest origin. Labeling with bromodeoxyuridine (BrdU) and detection of proliferating cell nuclear antigen (PCNA) expression shows that the mass in ephrin-A5(-/-) animals is mitotically active in embryonic and postnatal stages. CONCLUSIONS:Ephrin-A5 is a critical factor that regulates primary vitreous regression.
Authors: M B Reichel; R R Ali; F D'Esposito; A R Clarke; P J Luthert; S S Bhattacharya; D M Hunt Journal: Cell Death Differ Date: 1998-02 Impact factor: 15.828
Authors: Michal Sheleg; Carrie L Yochum; George C Wagner; Renping Zhou; Jason R Richardson Journal: Behav Brain Res Date: 2012-08-27 Impact factor: 3.332
Authors: Natalie E Freeman-Anderson; Yanbin Zheng; Amy C McCalla-Martin; Louise M Treanor; Yi D Zhao; Phillip M Garfin; Tong-Chuan He; Michelle N Mary; J Derek Thornton; Colleen Anderson; Melissa Gibbons; Raya Saab; Shannon H Baumer; John M Cunningham; Stephen X Skapek Journal: Development Date: 2009-06 Impact factor: 6.868
Authors: Toshihide Kurihara; Peter D Westenskow; Tim U Krohne; Edith Aguilar; Randall S Johnson; Martin Friedlander Journal: J Cell Biol Date: 2011-11-14 Impact factor: 10.539
Authors: Masaki Kato; Millan S Patel; Regis Levasseur; Ivan Lobov; Benny H-J Chang; Donald A Glass; Christine Hartmann; Lan Li; Tae-Ho Hwang; Cory F Brayton; Richard A Lang; Gerard Karsenty; Lawrence Chan Journal: J Cell Biol Date: 2002-04-15 Impact factor: 10.539
Authors: Michal Sheleg; Carrie L Yochum; Jason R Richardson; George C Wagner; Renping Zhou Journal: Behav Brain Res Date: 2015-03-06 Impact factor: 3.332