Literature DB >> 24549662

In vitro assay of pre-mRNA splicing in mammalian nuclear extract.

Maliheh Movassat1, William F Mueller, Klemens J Hertel.   

Abstract

The in vitro splicing assay is a valuable technique that can be used to study the mechanism and machinery involved in the splicing process. The ability to investigate various aspects of splicing and alternative splicing appears to be endless due to the flexibility of this assay. Here, we describe the tools and techniques necessary to carry out an in vitro splicing assay. Through the use of radiolabeled pre-mRNA and crude nuclear extract, spliced mRNAs can be purified and visualized by autoradiography for downstream analysis.

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Year:  2014        PMID： 24549662      PMCID： PMC4688300          DOI： 10.1007/978-1-62703-980-2_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

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Journal:  Genes Dev       Date:  1988-06       Impact factor: 11.361

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Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

6.  Better conditions for mammalian in vitro splicing provided by acetate and glutamate as potassium counterions.

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Journal:  Nucleic Acids Res       Date:  2000-01-15       Impact factor: 16.971

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Journal:  Cold Spring Harb Protoc       Date:  2010-06

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Journal:  Nature       Date:  1984 Mar 22-28       Impact factor: 49.962

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Authors:  A R Krainer; T Maniatis; B Ruskin; M R Green
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

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Authors:  P J Furdon; R Kole
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205
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  7 in total

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Authors:  Brandon E Aubol; Guowei Wu; Malik M Keshwani; Maliheh Movassat; Laurent Fattet; Klemens J Hertel; Xiang-Dong Fu; Joseph A Adams
Journal:  Mol Cell       Date:  2016-07-07       Impact factor: 17.970

2.  Transcription Factor GATA4 Regulates Cell Type-Specific Splicing Through Direct Interaction With RNA in Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitors.

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Journal:  Circulation       Date:  2022-08-08       Impact factor: 39.918

3.  Aberrant RNA methylation triggers recruitment of an alkylation repair complex.

Authors:  Ning Tsao; Joshua R Brickner; Rebecca Rodell; Adit Ganguly; Matthew Wood; Clement Oyeniran; Tanveer Ahmad; Hua Sun; Albino Bacolla; Lisheng Zhang; Valentina Lukinović; Jennifer M Soll; Brittany A Townley; Alexandre G Casanova; John A Tainer; Chuan He; Alessandro Vindigni; Nicolas Reynoird; Nima Mosammaparast
Journal:  Mol Cell       Date:  2021-10-21       Impact factor: 19.328

Review 4.  The Importance of a Genome-Wide Association Analysis in the Study of Alternative Splicing Mutations in Plants with a Special Focus on Maize.

Authors:  Zi-Chang Jia; Xue Yang; Xuan-Xuan Hou; Yong-Xin Nie; Jian Wu
Journal:  Int J Mol Sci       Date:  2022-04-11       Impact factor: 6.208

5.  Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay.

Authors:  Maliheh Movassat; Hossein Shenasa; Klemens J Hertel
Journal:  Methods Mol Biol       Date:  2017

6.  Development of an in vitro pre-mRNA splicing assay using plant nuclear extract.

Authors:  Mohammed Albaqami; Anireddy S N Reddy
Journal:  Plant Methods       Date:  2018-01-08       Impact factor: 4.993

7.  The influence of 4-thiouridine labeling on pre-mRNA splicing outcomes.

Authors:  Jessie A C Altieri; Klemens J Hertel
Journal:  PLoS One       Date:  2021-12-13       Impact factor: 3.240
  7 in total

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