Glycollate formation during the photoassimilation of acetate by Chlorella.

When (3)H-(14)C-acetate was supplied to Chlorella pyrenoidosa in the light, glycollic acid became rapidly labelled with tritium and (14)C. The [Formula: see text]ratio of glycollate was 10, whilst the ratio was 4 in the acetate added. Both (3)H and (14)C from acetate were present in glycollate before they were present in Calvin cycle intermediates, so that glycollate was not formed as a C2-fragment from the Calvin cycle.