| Literature DB >> 2454475 |
M Saleh1, R J Lang, P F Bartlett.
Abstract
The physiological effect of ligand binding to the Thy-1 molecule expressed on the neuronal cell surface was examined. Sensory neurons obtained from dorsal root ganglia of 2-day-old CBA/CAH mice (Thy-1.2) were cultured in vitro for 8 days, and then patch-clamp recordings were obtained of calcium currents. It was found that binding of an anti-Thy-1.2 monoclonal antibody to neurons expressing the Thy-1.2 moiety increased the amplitude of a low-threshold transient calcium current. Monoclonal antibodies directed against the Thy-1.1 molecule or a neuronal surface ganglioside (A2B5) had no effect on this calcium current in Thy-1.2-bearing cells, nor did they interfere with subsequent activation by the anti-Thy-1.2 monoclonal antibody. These results demonstrate that ligand binding to the Thy-1 molecule transduces a physiological signal in sensory neurons by increasing a voltage-activated calcium current.Entities:
Mesh：
Substances：
Year: 1988 PMID： 2454475 PMCID： PMC280467 DOI： 10.1073/pnas.85.12.4543
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205