Literature DB >> 2454178

Improved resolution of flow cytometric measurements of Hoechst- and chromomycin-A3-stained human chromosomes after addition of citrate and sulfite.

G van den Engh1, B Trask, P Lansdorp, J Gray.   

Abstract

The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high-resolution measurements to be made and evaluated reliably from clinical samples.

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Year:  1988        PMID： 2454178     DOI： 10.1002/cyto.990090313

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  11 in total

1.  Inheritance of chromosome heteromorphisms analyzed by high-resolution bivariate flow karyotyping.

Authors:  B Trask; G van den Engh; J W Gray
Journal:  Am J Hum Genet       Date:  1989-11       Impact factor: 11.025

2.  The bovine bivariate flow karyotype and peak identification by chromosome painting with PCR-generated probes.

Authors:  A Schmitz; A Oustry; B Chaput; I Bahri-Darwich; M Yerle; D Millan; G Frelat; E P Cribiu
Journal:  Mamm Genome       Date:  1995-06       Impact factor: 2.957

3.  Chromosomal localization of the porcine skeletal muscle calpain gene.

Authors:  G P Briley; P K Riggs; J E Womack; D L Hancock; C A Bidwell
Journal:  Mamm Genome       Date:  1996-03       Impact factor: 2.957

4.  An Xp22 microdeletion associated with ocular albinism and ichthyosis: approximation of breakpoints and estimation of deletion size by using cloned DNA probes and flow cytometry.

Authors:  R E Schnur; B J Trask; G van den Engh; H H Punnett; M Kistenmacher; M A Tomeo; R E Naids; R L Nussbaum
Journal:  Am J Hum Genet       Date:  1989-11       Impact factor: 11.025

5.  Chromosome heteromorphism quantified by high-resolution bivariate flow karyotyping.

Authors:  B Trask; G van den Engh; B Mayall; J W Gray
Journal:  Am J Hum Genet       Date:  1989-11       Impact factor: 11.025

6.  Characterization of three de novo derivative chromosomes 16 by "reverse chromosome painting" and molecular analysis.

Authors:  K A Rack; P C Harris; A B MacCarthy; R Boone; H Raynham; M McKinley; M Fitchett; C M Towe; P Rudd; J A Armour
Journal:  Am J Hum Genet       Date:  1993-05       Impact factor: 11.025

7.  Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays.

Authors:  Susan M Gribble; Bee Ling Ng; Elena Prigmore; Tomas Fitzgerald; Nigel P Carter
Journal:  Nat Protoc       Date:  2009-11-05       Impact factor: 13.491

8.  Xp21 contiguous gene syndromes: deletion quantitation with bivariate flow karyotyping allows mapping of patient breakpoints.

Authors:  E R McCabe; J A Towbin; G van den Engh; B J Trask
Journal:  Am J Hum Genet       Date:  1992-12       Impact factor: 11.025

9.  Choroideremia and deafness with stapes fixation: a contiguous gene deletion syndrome in Xq21.

Authors:  D E Merry; J G Lesko; D M Sosnoski; R A Lewis; M Lubinsky; B Trask; G van den Engh; F S Collins; R L Nussbaum
Journal:  Am J Hum Genet       Date:  1989-10       Impact factor: 11.025

10.  Use of fluorescent sequence-specific polyamides to discriminate human chromosomes by microscopy and flow cytometry.

Authors:  Melanie P Gygi; Mark D Ferguson; Heather C Mefford; Kevin P Lund; Christine O'Day; Peiwen Zhou; Cynthia Friedman; Ger van den Engh; Mark L Stolowitz; Barbara J Trask
Journal:  Nucleic Acids Res       Date:  2002-07-01       Impact factor: 16.971
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