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Literature DB >> 2453501

Human renal clear cell carcinoma: establishment and characterization of a new cell line (G-2101).

P H Gumerlock¹, B F Edwards, A D Deitch, F J Meyers.

Abstract

A human cell line has been established from a renal adenocarcinoma rib metastasis of a 58-y-old male. This cell line has been maintained in continuous culture for 20 mo. through more than 50 passages. It displays simultaneous expression of the intermediate filaments cytokeratin and vimentin. Flow cytometric analysis of DNA content reveals a major hyperdiploid population.

Entities: Disease Gene Species

Mesh：

Substances：
Vimentin
Keratins

Year: 1988 PMID： 2453501 DOI： 10.1007/bf02628494

Source DB: PubMed Journal: In Vitro Cell Dev Biol ISSN： 0883-8364

29 in total

1. Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes.

Authors: M B BORNSTEIN
Journal: Lab Invest Date: 1958 Mar-Apr Impact factor: 5.662

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

Review 4. Application of immunohistochemical methods in the diagnosis of malignant disease.

Authors: A Imam; C R Taylor
Journal: Cancer Invest Date: 1985 Impact factor: 2.176

5. Isolation and characterization of intermediate filaments.

Authors: P Steinert; R Zackroff; M Aynardi-Whitman; R D Goldman
Journal: Methods Cell Biol Date: 1982 Impact factor: 1.441

Review 6. The role of flow cytometry in urologic disease.

Authors: R W deVere White; A D Deitch
Journal: Semin Urol Date: 1985-05

Review 7. Intermediate filament proteins in the study of tumor heterogeneity: an in-depth study of tumors of the urinary and respiratory tracts.

Authors: F C Ramaekers; O Moesker; A Huysmans; G Schaart; G Westerhof; S S Wagenaar; C J Herman; G P Vooijs
Journal: Ann N Y Acad Sci Date: 1985 Impact factor: 5.691

Human renal clear cell carcinoma: establishment and characterization of a new cell line (G-2101).

1. Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Review 4. Application of immunohistochemical methods in the diagnosis of malignant disease.

5. Isolation and characterization of intermediate filaments.

Review 6. The role of flow cytometry in urologic disease.

Review 7. Intermediate filament proteins in the study of tumor heterogeneity: an in-depth study of tumors of the urinary and respiratory tracts.

8. In vitro cultivation of human renal cell cancer. II. Characterization of cell lines.

9. Human renal cell carcinoma: establishment and characterization of two new cell lines.

10. Characterization of an established cell line from human renal carcinoma.

1. Oxalate transport in cultured porcine renal epithelial cells.