Javier Ramos-Torrecillas1, Elvira de Luna-Bertos, Francisco J Manzano-Moreno, Olga García-Martínez, Concepción Ruiz. 1. Javier Ramos-Torrecillas, PhD, is a member of the Department of Nursing, Faculty of Health Sciences, University of Granada, Spain. Elvira de Luna-Bertos, PhD, is a member of the Department of Nursing, Faculty of Health Sciences, University of Granada, Spain. Francisco J. Manzano-Moreno, MS, is a PhD student in the Department of Odontology and Stomatology, Faculty of Odontology, University of Granada, Spain. Olga García-Martínez, PhD, is a member of the Department of Nursing, Faculty of Health Sciences, University of Granada, Spain. Concepción Ruiz, PhD, is a professor in the Department of Nursing, Faculty of Health Sciences, University of Granada, Spain.
Abstract
OBJECTIVE: The purpose of this study was to compare the proliferation, morphology, and antigenic expression of human fibroblast-like cells between primary cultures treated with platelet-rich plasma (PRP) or fetal bovine serum (FBS) as the growth factor source. DESIGN: Cells from human gingival tissue samples obtained from healthy volunteers during oral surgery were studied. Isolated cells were cultured in media supplemented with 10% PRP or FBS. Platelet-rich plasma was prepared from the venous blood of each patient. The authors studied short- and long-term cell cultures in the presence of PRP or FBS as the sole growth factor source in order to determine (a) cell growth rate, by MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay; (b) cell morphology, by electronic microscopy; and (c) antigenic expression, by flow cytometry and confocal microscopy. RESULTS: In short-term cultures, the cell growth rate was higher with PRP versus FBS treatment. No differences in morphology or expression of vimentin, fibronectin, or α-actin antigens were observed between PRP and FBS cultures. In long-term cultures, PRP and FBS did not significantly differ in cell growth rate but differed in morphology and in the expression of vimentin, fibronectin, and α-actin. CONCLUSION: The PRP enhances cell proliferation over the short term and induces cell differentiation of fibroblast-like cells to myofibroblast-like cells over the long term, suggesting that fibroblast differentiation to myofibroblasts may underlie the action mechanism of PRP in soft tissue regeneration.
OBJECTIVE: The purpose of this study was to compare the proliferation, morphology, and antigenic expression of human fibroblast-like cells between primary cultures treated with platelet-rich plasma (PRP) or fetal bovine serum (FBS) as the growth factor source. DESIGN: Cells from human gingival tissue samples obtained from healthy volunteers during oral surgery were studied. Isolated cells were cultured in media supplemented with 10% PRP or FBS. Platelet-rich plasma was prepared from the venous blood of each patient. The authors studied short- and long-term cell cultures in the presence of PRP or FBS as the sole growth factor source in order to determine (a) cell growth rate, by MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay; (b) cell morphology, by electronic microscopy; and (c) antigenic expression, by flow cytometry and confocal microscopy. RESULTS: In short-term cultures, the cell growth rate was higher with PRP versus FBS treatment. No differences in morphology or expression of vimentin, fibronectin, or α-actin antigens were observed between PRP and FBS cultures. In long-term cultures, PRP and FBS did not significantly differ in cell growth rate but differed in morphology and in the expression of vimentin, fibronectin, and α-actin. CONCLUSION: The PRP enhances cell proliferation over the short term and induces cell differentiation of fibroblast-like cells to myofibroblast-like cells over the long term, suggesting that fibroblast differentiation to myofibroblasts may underlie the action mechanism of PRP in soft tissue regeneration.
Authors: Eduardo Anitua; Mar Zalduendo; Maria Troya; Mohammad H Alkhraisat; Leticia Alejandra Blanco-Antona Journal: Int J Mol Sci Date: 2022-06-11 Impact factor: 6.208
Authors: Francisco Javier Manzano-Moreno; Rebeca Illescas-Montes; Lucia Melguizo-Rodriguez; Victor J Costela-Ruiz; Olga García-Martínez; Concepción Ruiz; Javier Ramos-Torrecillas Journal: Int J Med Sci Date: 2019-10-21 Impact factor: 3.738