AIMS: To investigate early alterations on bone mineral density (BMD) and RANK, RANKL and OPG mRNA expression in peripheral blood leukocytes (PBL) in children and adolescents with type 1 diabetes (T1D) and the relationship with glycemic control and bone biomarkers. METHODS: This cross-sectional study included 75 children and adolescents with T1D and 100 individuals without diabetes (normoglycemic-NG) aged 6-20 years old. T1D individuals were considered to have good (T1DG) or poor (T1DP) glycemic control according to the values of HbA1c. Phosphorus, magnesium, total and ionized calcium, osteocalcin, alkaline phosphatase and tartaric-resistant acid phosphatase (TRAP) values were determined in blood samples. BMD was measured by DEXA. RANK, RANKL and OPG mRNA expression was measured in PBL by real-time PCR. RESULTS: Osteocalcin values were decreased in diabetic groups in comparison to NG group (p<0.05), and a negative correlation with both serum glucose (r=-0.265, p<0.01) and Hb1Ac (r=-0.252, p<0.01) in T1D group was found. BMD was lower in diabetic groups in comparison with NG group (p<0.05) and a negative correlation was observed between BMD and both serum glucose (r=-0.357, p<0.01) and HbA1c (r=-0.351, p<0.01) in T1D group. OPG mRNA expression was significantly increased in T1D and T1DP groups in comparison with NG group (p<0.05). In conclusion, children and adolescents with early onset T1D presented low bone mineral density associated to unsatisfactory glycemic control, increased OPG mRNA expression and low osteocalcin concentration.
AIMS: To investigate early alterations on bone mineral density (BMD) and RANK, RANKL and OPG mRNA expression in peripheral blood leukocytes (PBL) in children and adolescents with type 1 diabetes (T1D) and the relationship with glycemic control and bone biomarkers. METHODS: This cross-sectional study included 75 children and adolescents with T1D and 100 individuals without diabetes (normoglycemic-NG) aged 6-20 years old. T1D individuals were considered to have good (T1DG) or poor (T1DP) glycemic control according to the values of HbA1c. Phosphorus, magnesium, total and ionizedcalcium, osteocalcin, alkaline phosphatase and tartaric-resistant acid phosphatase (TRAP) values were determined in blood samples. BMD was measured by DEXA. RANK, RANKL and OPG mRNA expression was measured in PBL by real-time PCR. RESULTS:Osteocalcin values were decreased in diabetic groups in comparison to NG group (p<0.05), and a negative correlation with both serum glucose (r=-0.265, p<0.01) and Hb1Ac (r=-0.252, p<0.01) in T1D group was found. BMD was lower in diabetic groups in comparison with NG group (p<0.05) and a negative correlation was observed between BMD and both serum glucose (r=-0.357, p<0.01) and HbA1c (r=-0.351, p<0.01) in T1D group. OPG mRNA expression was significantly increased in T1D and T1DP groups in comparison with NG group (p<0.05). In conclusion, children and adolescents with early onset T1D presented low bone mineral density associated to unsatisfactory glycemic control, increased OPG mRNA expression and low osteocalcin concentration.
Authors: Mishaela R Rubin; Ian H de Boer; Jye-Yu C Backlund; Valerie Arends; Rose Gubitosi-Klug; Amisha Wallia; Naina Sinha Gregory; Annette Barnie; Andrew J Burghardt; John M Lachin; Barbara H Braffett; Ann V Schwartz Journal: J Clin Endocrinol Metab Date: 2022-05-17 Impact factor: 6.134
Authors: C Tsentidis; D Gourgiotis; L Kossiva; A Doulgeraki; A Marmarinos; A Galli-Tsinopoulou; K Karavanaki Journal: Osteoporos Int Date: 2015-11-20 Impact factor: 4.507
Authors: Raul Hernandes Bortolin; Bento João da Graça Azevedo Abreu; Marcela Abbott Galvão Ururahy; Karla Simone Costa de Souza; João Felipe Bezerra; Melina Bezerra Loureiro; Flávio Santos da Silva; Dáfiny Emanuele da Silva Marques; Angélica Amanda de Sousa Batista; Gisele Oliveira; André Ducati Luchessi; Valéria Morgiana Gualberto Duarte Moreira Lima; Carlos Eduardo Saraiva Miranda; Marcus Vinicius Lia Fook; Maria das Graças Almeida; Luciana Augusto de Rezende; Adriana Augusto de Rezende Journal: PLoS One Date: 2015-05-01 Impact factor: 3.240