| Literature DB >> 24529374 |
Astrid Gadeyne1, Clara Sánchez-Rodríguez2, Steffen Vanneste1, Simone Di Rubbo1, Henrik Zauber2, Kevin Vanneste1, Jelle Van Leene1, Nancy De Winne1, Dominique Eeckhout1, Geert Persiau1, Eveline Van De Slijke1, Bernard Cannoot1, Leen Vercruysse1, Jonathan R Mayers3, Maciek Adamowski4, Urszula Kania4, Matthias Ehrlich2, Alois Schweighofer5, Tijs Ketelaar6, Steven Maere1, Sebastian Y Bednarek3, Jiří Friml4, Kris Gevaert7, Erwin Witters8, Eugenia Russinova1, Staffan Persson9, Geert De Jaeger1, Daniël Van Damme10.
Abstract
Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.Mesh：
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Year: 2014 PMID： 24529374 DOI： 10.1016/j.cell.2014.01.039
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582