Enhanced therapeutic efficacy on lymph node metastasis by the use of peplomycin adsorbed on small activated carbon particles.

Mice into which 5 x 10(5) MH134 tumor cells had been inoculated subcutaneously opposite the foot-pad of the left hind paw on day 0, received subcutaneous injection of 0.25 mg/mouse of peplomycin in the form of PEP-CH (peplomycin adsorbed on activated carbon particles) or peplomycin aqueous solution into the foot-pad of the left hind paw on day 10. The left popliteal lymph nodes were transferred intraperitoneally to normal mice (the recipients) on day 14. In the PEP-CH group the survival time of the recipients was statistically significantly longer and the transferred tumor cell number estimated by calibration curve was markedly smaller in comparison with those in the other treatment groups.