| Literature DB >> 24525041 |
Jianyong Zheng1, Xianfeng Fu1, Xiangxian Ying1, Yinjun Zhang1, Zhao Wang2.
Abstract
A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments.Entities:
Keywords: Derivatization; High-throughput screening; Lipase; MBTH; Synthetic activity
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Year: 2014 PMID： 24525041 DOI： 10.1016/j.ab.2014.02.001
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365