Literature DB >> 2452439

Agrobacterium tumefaciens virE operon encodes a single-stranded DNA-binding protein.

A Das1.   

Abstract

The virulence (vir) genes of Agrobacterium tumefaciens Ti plasmid are essential for transformation of plant cells. Overproduction of a virE-encoded gene product in Escherichia coli was achieved by construction of an operon fusion with the E. coli tryptophan (trp) operon. The virE2 gene product in E. coli partitioned into the insoluble membrane fraction. The protein was solubilized by treatment with 4 M urea at 0 degree C. DNA-protein binding experiments showed that a strong single-stranded (ss) DNA-binding activity was present in protein fractions containing the virE2 gene product. The binding was highly specific with little or no binding observed with either double-stranded DNA or ssRNA. No significant binding to Ti plasmid DNA sequences was observed. Protein blotting studies indicated that the ssDNA-binding activity was associated with the 68-kDa virE2 polypeptide.

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Year:  1988        PMID： 2452439      PMCID： PMC280112          DOI： 10.1073/pnas.85.9.2909

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  DNA binding properties of the T4 DNA helix-destabilizing protein. A calorimetric study.

Authors:  K R Williams; L O Sillerud; D E Schafer; W H Konigsberg
Journal:  J Biol Chem       Date:  1979-07-25       Impact factor: 5.157

2.  Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.

Authors:  W K Miskimins; M P Roberts; A McClelland; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

6.  DNA from the A6S/2 crown gall tumor contains scrambled Ti-plasmid sequences near its junctions with plant DNA.

Authors:  R B Simpson; P J O'Hara; W Kwok; A L Montoya; C Lichtenstein; M P Gordon; E W Nester
Journal:  Cell       Date:  1982-07       Impact factor: 41.582

7.  Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria.

Authors:  T J Schmidhauser; D R Helinski
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

8.  Transfer of Agrobacterium DNA to Plants Requires a T-DNA Border But Not the virE Locus.

Authors:  R C Gardner; V C Knauf
Journal:  Science       Date:  1986-02-14       Impact factor: 47.728

9.  Characterization of the virE operon of the Agrobacterium Ti plasmid pTiA6.

Authors:  S C Winans; P Allenza; S E Stachel; K E McBride; E W Nester
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

10.  The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens.

Authors:  S E Stachel; E W Nester
Journal:  EMBO J       Date:  1986-07       Impact factor: 11.598
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  52 in total

1.  The six functions of Agrobacterium VirE2.

Authors:  D V Ward; P C Zambryski
Journal:  Proc Natl Acad Sci U S A       Date:  2001-01-16       Impact factor: 11.205

2.  VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity.

Authors:  T Tzfira; M Vaidya; V Citovsky
Journal:  EMBO J       Date:  2001-07-02       Impact factor: 11.598

Review 3.  Two-way chemical signaling in Agrobacterium-plant interactions.

Authors:  S C Winans
Journal:  Microbiol Rev       Date:  1992-03

4.  Activation of the T-DNA transfer process in Agrobacterium results in the generation of a T-strand-protein complex: Tight association of VirD2 with the 5' ends of T-strands.

Authors:  E A Howard; B A Winsor; G De Vos; P Zambryski
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

5.  Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells.

Authors:  J R Zupan; V Citovsky; P Zambryski
Journal:  Proc Natl Acad Sci U S A       Date:  1996-03-19       Impact factor: 11.205

6.  Plant chromosome/marker gene fusion assay for study of normal and truncated T-DNA integration events.

Authors:  L Herman; A Jacobs; M Van Montagu; A Depicker
Journal:  Mol Gen Genet       Date:  1990-11

Review 7.  Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria.

Authors:  P J Christie
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

8.  Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation.

Authors:  F Dürrenberger; A Crameri; B Hohn; Z Koukolíková-Nicola
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

9.  Activity of the Agrobacterium T-DNA transfer machinery is affected by virB gene products.

Authors:  J E Ward; E M Dale; A N Binns
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

10.  Site-specific integration of Agrobacterium tumefaciens T-DNA via double-stranded intermediates.

Authors:  Tzvi Tzfira; Leah Renée Frankman; Manjusha Vaidya; Vitaly Citovsky
Journal:  Plant Physiol       Date:  2003-10-09       Impact factor: 8.340
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