Literature DB >> 2452229

Electrophoretic and immunochemical study of the lipopolysaccharides produced by chemostat-grown Escherichia coli O157.

K L Dodds1, M B Perry, I J McDonald.   

Abstract

Two chemically different O-polysaccharides, a low molecular mass form of LPS and core LPS produced by chemostat-grown E. coli O157, were analysed by SDS-PAGE, silver staining and immunoblotting. The reactivities of the different O-polysaccharides with antisera prepared against E. coli O157 grown in batch culture, Salmonella O30 or Brucella abortus were very similar, showing that the O-polysaccharides share at least some antigenic determinants. The reactions of the low molecular mass LPS with the antisera indicated it was semi-rough LPS having one repeat unit of the O-polysaccharide attached to core LPS.

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Year:  1987        PMID: 2452229     DOI: 10.1099/00221287-133-9-2679

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN: 0022-1287


  5 in total

1.  Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157.

Authors:  R B Westerman; Y He; J E Keen; E T Littledike; J Kwang
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

2.  Neutralizing antibodies to Escherichia coli Vero cytotoxin 1 and antibodies to O157 lipopolysaccharide in healthy farm family members and urban residents.

Authors:  D Reymond; R P Johnson; M A Karmali; M Petric; M Winkler; S Johnson; K Rahn; S Renwick; J Wilson; R C Clarke; J Spika
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

3.  Development of a blocking enzyme-linked immunosorbent assay for detection of serum antibodies to O157 antigen of Escherichia coli.

Authors:  W Laegreid; M Hoffman; J Keen; R Elder; J Kwang
Journal:  Clin Diagn Lab Immunol       Date:  1998-03

4.  Identification of a rough strain of Escherichia coli O157:H7 that produces no detectable O157 antigen.

Authors:  P Feng; R C Sandlin; C H Park; R A Wilson; M Nishibuchi
Journal:  J Clin Microbiol       Date:  1998-08       Impact factor: 5.948

5.  Serum antibody responses of cattle following experimental infection with Escherichia coli O157:H7.

Authors:  R P Johnson; W C Cray; S T Johnson
Journal:  Infect Immun       Date:  1996-05       Impact factor: 3.441

  5 in total

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