Literature DB >> 2452094

Specific radioimmunoprecipitation of histone H2A antigens by protein A conjugated sepharose.

F J Ruder1, M Frasch, W Büsen.   

Abstract

A modified radioimmunoprecipitation technique is described which allows the specific detection of histone H2A antigens. The technique circumvents unspecific binding of histones to the bacterial adsorbent.

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Year:  1988        PMID: 2452094     DOI: 10.1007/bf01961278

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  6 in total

1.  Appearance of two maternally directed histone H2A variants precedes zygotic ubiquitination of H2A in early embryogenesis of Sciara coprophila (Diptera).

Authors:  F J Ruder; M Frasch; T C Mettenleiter; W Büsen
Journal:  Dev Biol       Date:  1987-08       Impact factor: 3.582

2.  A rapid sensitive assay for specific protein synthesis in cells and in cell-free translations: use of Staphylococcus aureus as an adsorbent for immune complexes.

Authors:  R D Ivarie; P P Jones
Journal:  Anal Biochem       Date:  1979-08       Impact factor: 3.365

3.  "Protein A" from Staphylococcus aureus. 3. Reaction with rabbit gamma-globulin.

Authors:  A Forsgren; J Sjöquist
Journal:  J Immunol       Date:  1967-07       Impact factor: 5.422

4.  "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin.

Authors:  A Forsgren; J Sjöquist
Journal:  J Immunol       Date:  1966-12       Impact factor: 5.422

5.  Highly sensitive immunoadsorption procedure for detection of low-abundance proteins.

Authors:  E J Platt; K Karlsen; A Lopez-Valdivieso; P W Cook; G L Firestone
Journal:  Anal Biochem       Date:  1986-07       Impact factor: 3.365

6.  Precipitation of radiolabeled antigen-antibody complexes with protein A-containing Staphylococcus aureus.

Authors:  M J Brunda; P Minden; T R Sharpton; J K McClatchy; R S Farr
Journal:  J Immunol       Date:  1977-07       Impact factor: 5.422

  6 in total

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