Caimei Zhang1, Biyi Chen, Ang Guo, Yanqi Zhu, Jordan D Miller, Shan Gao, Can Yuan, William Kutschke, Kathy Zimmerman, Robert M Weiss, Xander H T Wehrens, Jiang Hong, Frances L Johnson, Luis F Santana, Mark E Anderson, Long-Sheng Song. 1. Division of Cardiovascular Medicine, Department of Internal Medicine (C.Z., B.C., A.G., Y.Z., S.G., W.K., R.M.W., F.L.J., M.E.A., L.-S.S.) and Department of Molecular Physiology and Biophysics (M.E.A.), University of Iowa Carver College of Medicine, Iowa City, IA; Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China (Y.Z., J.H.); Division of Cardiovascular Surgery, Mayo Clinic, Rochester, MN (J.D.M.); Department of Pharmacology, College of Basic Medicine, Anhui Medical University, Hefei, China (S.G.); Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA (C.Y., L.F.S.); Department of Veterans Affairs Medical Center, Iowa City, IA (K.Z.); and Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX (X.H.T.W.).
Abstract
BACKGROUND: Cardiac dysfunction in failing hearts of human patients and animal models is associated with both microtubule densification and transverse-tubule (T-tubule) remodeling. Our objective was to investigate whether microtubule densification contributes to T-tubule remodeling and excitation-contraction coupling dysfunction in heart disease. METHODS AND RESULTS: In a mouse model of pressure overload-induced cardiomyopathy by transaortic banding, colchicine, a microtubule depolymerizer, significantly ameliorated T-tubule remodeling and cardiac dysfunction. In cultured cardiomyocytes, microtubule depolymerization with nocodazole or colchicine profoundly attenuated T-tubule impairment, whereas microtubule polymerization/stabilization with taxol accelerated T-tubule remodeling. In situ immunofluorescence of heart tissue sections demonstrated significant disorganization of junctophilin-2 (JP2), a protein that bridges the T-tubule and sarcoplasmic reticulum membranes, in transaortic banded hearts as well as in human failing hearts, whereas colchicine injection significantly preserved the distribution of JP2 in transaortic banded hearts. In isolated mouse cardiomyocytes, prolonged culture or treatment with taxol resulted in pronounced redistribution of JP2 from T-tubules to the peripheral plasma membrane, without changing total JP2 expression. Nocodazole treatment antagonized JP2 redistribution. Moreover, overexpression of a dominant-negative mutant of kinesin 1, a microtubule motor protein responsible for anterograde trafficking of proteins, protected against JP2 redistribution and T-tubule remodeling in culture. Finally, nocodazole treatment improved Ca(2+) handling in cultured myocytes by increasing the amplitude of Ca(2+) transients and reducing the frequency of Ca(2+) sparks. CONCLUSION: Our data identify a mechanistic link between microtubule densification and T-tubule remodeling and reveal microtubule-mediated JP2 redistribution as a novel mechanism for T-tubule disruption, loss of excitation-contraction coupling, and heart failure.
BACKGROUND:Cardiac dysfunction in failing hearts of humanpatients and animal models is associated with both microtubule densification and transverse-tubule (T-tubule) remodeling. Our objective was to investigate whether microtubule densification contributes to T-tubule remodeling and excitation-contraction coupling dysfunction in heart disease. METHODS AND RESULTS: In a mouse model of pressure overload-induced cardiomyopathy by transaortic banding, colchicine, a microtubule depolymerizer, significantly ameliorated T-tubule remodeling and cardiac dysfunction. In cultured cardiomyocytes, microtubule depolymerization with nocodazole or colchicine profoundly attenuated T-tubule impairment, whereas microtubule polymerization/stabilization with taxol accelerated T-tubule remodeling. In situ immunofluorescence of heart tissue sections demonstrated significant disorganization of junctophilin-2 (JP2), a protein that bridges the T-tubule and sarcoplasmic reticulum membranes, in transaortic banded hearts as well as in human failing hearts, whereas colchicine injection significantly preserved the distribution of JP2 in transaortic banded hearts. In isolated mouse cardiomyocytes, prolonged culture or treatment with taxol resulted in pronounced redistribution of JP2 from T-tubules to the peripheral plasma membrane, without changing total JP2 expression. Nocodazole treatment antagonized JP2 redistribution. Moreover, overexpression of a dominant-negative mutant of kinesin 1, a microtubule motor protein responsible for anterograde trafficking of proteins, protected against JP2 redistribution and T-tubule remodeling in culture. Finally, nocodazole treatment improved Ca(2+) handling in cultured myocytes by increasing the amplitude of Ca(2+) transients and reducing the frequency of Ca(2+) sparks. CONCLUSION: Our data identify a mechanistic link between microtubule densification and T-tubule remodeling and reveal microtubule-mediated JP2 redistribution as a novel mechanism for T-tubule disruption, loss of excitation-contraction coupling, and heart failure.
Authors: Louise A Aquila-Pastir; Nicholas R DiPaola; Rosalia G Matteo; Nicholas G Smedira; Patrick M McCarthy; Christine Schomisch Moravec Journal: J Mol Cell Cardiol Date: 2002-11 Impact factor: 5.000
Authors: M Koide; M Hamawaki; T Narishige; H Sato; S Nemoto; G DeFreyte; M R Zile; I V Cooper G; B A Carabello Journal: Circulation Date: 2000-08-29 Impact factor: 29.690
Authors: William E Louch; Virginie Bito; Frank R Heinzel; Regina Macianskiene; Johan Vanhaecke; Willem Flameng; Kanigula Mubagwa; Karin R Sipido Journal: Cardiovasc Res Date: 2004-04-01 Impact factor: 10.787
Authors: Ang Guo; Xiaoying Zhang; Venkat Ramesh Iyer; Biyi Chen; Caimei Zhang; William J Kutschke; Robert M Weiss; Clara Franzini-Armstrong; Long-Sheng Song Journal: Proc Natl Acad Sci U S A Date: 2014-08-04 Impact factor: 11.205