Literature DB >> 2451829

Nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kDa in chicken embryo fibroblasts.

M E Linder1, J G Burr.   

Abstract

We have used anti-phosphotyrosine antibodies to identify a large number of tyrosine phosphoproteins in Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts. Most of these proteins were found in the 100,000 X g supernatant when cells were separated into soluble and particulate fractions; however, one group of proteins, of 115-120 kDa, was found in the particulate fraction. The phosphotyrosine content of the 115- to 120-kDa proteins was greatly reduced in chicken embryo fibroblasts infected with mutants of RSV (NY314 and SD10) encoding nonmyristoylated forms of the viral src gene product that do not associate with cellular membranes. Since RSV mutants NY314 and SD10 do not transform cells, phosphorylation of this group of 115- to 120-kDa membrane proteins may be related to the process of transformation.

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Year:  1988        PMID： 2451829      PMCID： PMC280047          DOI： 10.1073/pnas.85.8.2608

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  34 in total

Review 1.  Protein-tyrosine kinases.

Authors:  T Hunter; J A Cooper
Journal:  Annu Rev Biochem       Date:  1985       Impact factor: 23.643

2.  The absence of myristic acid decreases membrane binding of p60src but does not affect tyrosine protein kinase activity.

Authors:  J E Buss; M P Kamps; K Gould; B M Sefton
Journal:  J Virol       Date:  1986-05       Impact factor: 5.103

3.  Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins.

Authors:  J K Klarlund
Journal:  Cell       Date:  1985-07       Impact factor: 41.582

4.  Fine structural mapping of a critical NH2-terminal region of p60src.

Authors:  D Pellman; E A Garber; F R Cross; H Hanafusa
Journal:  Proc Natl Acad Sci U S A       Date:  1985-03       Impact factor: 11.205

5.  Myristic acid, a rare fatty acid, is the lipid attached to the transforming protein of Rous sarcoma virus and its cellular homolog.

Authors:  J E Buss; B M Sefton
Journal:  J Virol       Date:  1985-01       Impact factor: 5.103

6.  Rous sarcoma virus transforming protein lacking myristic acid phosphorylates known polypeptide substrates without inducing transformation.

Authors:  M P Kamps; J E Buss; B M Sefton
Journal:  Cell       Date:  1986-04-11       Impact factor: 41.582

7.  Direct identification of phosphotyrosine-containing proteins in some retrovirus-transformed cells by use of anti-phosphotyrosine antibody.

Authors:  J Seki; M K Owada; N Sakato; H Fujio
Journal:  Cancer Res       Date:  1986-02       Impact factor: 12.701

8.  Myristic acid is attached to the transforming protein of Rous sarcoma virus during or immediately after synthesis and is present in both soluble and membrane-bound forms of the protein.

Authors:  J E Buss; M P Kamps; B M Sefton
Journal:  Mol Cell Biol       Date:  1984-12       Impact factor: 4.272

9.  Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation.

Authors:  M P Kamps; J E Buss; B M Sefton
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

10.  Amino terminal myristylation of the protein kinase p60src, a retroviral transforming protein.

Authors:  A M Schultz; L E Henderson; S Oroszlan; E A Garber; H Hanafusa
Journal:  Science       Date:  1985-01-25       Impact factor: 47.728
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  25 in total

1.  Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases.

Authors:  S B Kanner; A B Reynolds; R R Vines; J T Parsons
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

2.  Glycoprotein tyrosine phosphorylation in Rous sarcoma virus-transformed chicken embryo fibroblasts.

Authors:  L M Kozma; A B Reynolds; M J Weber
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

3.  Phosphorylation and activation of epidermal growth factor receptors in cells transformed by the src oncogene.

Authors:  W J Wasilenko; D M Payne; D L Fitzgerald; M J Weber
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272

4.  Transformation by pp60src or stimulation of cells with epidermal growth factor induces the stable association of tyrosine-phosphorylated cellular proteins with GTPase-activating protein.

Authors:  A H Bouton; S B Kanner; R R Vines; H C Wang; J B Gibbs; J T Parsons
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

5.  Identification and characterization of a novel cytoskeleton-associated pp60src substrate.

Authors:  H Wu; A B Reynolds; S B Kanner; R R Vines; J T Parsons
Journal:  Mol Cell Biol       Date:  1991-10       Impact factor: 4.272

6.  Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120-kDa protein.

Authors:  J L Guan; J E Trevithick; R O Hynes
Journal:  Cell Regul       Date:  1991-11

7.  A glycoprotein in the plasma membrane matrix as a major potential substrate of p60v-src.

Authors:  M Hamaguchi; M Matsuda; H Hanafusa
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

8.  Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene.

Authors:  A B Reynolds; D J Roesel; S B Kanner; J T Parsons
Journal:  Mol Cell Biol       Date:  1989-02       Impact factor: 4.272

9.  CDC37 is required for p60v-src activity in yeast.

Authors:  B Dey; J J Lightbody; F Boschelli
Journal:  Mol Biol Cell       Date:  1996-09       Impact factor: 4.138

10.  Ornithine decarboxylase- and ras-induced cell transformations: reversal by protein tyrosine kinase inhibitors and role of pp130CAS.

Authors:  M Auvinen; A Paasinen-Sohns; H Hirai; L C Andersson; E Hölttä
Journal:  Mol Cell Biol       Date:  1995-12       Impact factor: 4.272
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