Ivan Kostić1, Dragan Mihailović2, Stevo Najman3, Sanja Stojanović3, Milena Kostić4. 1. Institute of Biology and Human Genetics, Faculty of Medicine, University of Nis, Serbia. ivankostic@sbb.rs 2. Institute of Pathology, Faculty of Medicine, University of Nis, Serbia. 3. Institute of Biology and Human Genetics, Faculty of Medicine, University of Nis, Serbia. 4. Department of Prosthodontics, Clinic of Dentistry, Nis, Serbia.
Abstract
UNLABELLED: BACKROUND/AIM: Retraction agents for temporary vertical and lateral suppression of gingival tissue as well as bleeding control and fluid flow in the gingival sulcus are expected to have maximal efficiency without irreversible damage of local tissue and adverse systemic effects. The research started from the assumption that tetrahydrozoline is a biologically more acceptable means of gingival retraction than commercially available preparations. The aim of the study was to comparatively analyse the inflammatory effects of different retraction materials and tetrahydrozoline. METHODS: The effect of retraction liquid on the basis of aluminum chloride and epinephrine and tetrahydrozoline hydrochloride on gingival tissue of rabbits was investigated. The application time in the rabbit's gingival sulcus was 7 minutes. Tissue biopsy was performed after an hour, a day, and 7 and 30 days. Tissue preparations were analyzed under a microscope. RESULTS: The obtained results indicate a reversible damage of gingival tissues as a result of local application of aluminum chloride- and epinephrine-based retraction agents. Their use led to acute inflammatory response after an observation period of 1 and 7 days. After 30 days reparation of damaged tissue was observed. The use of tetrahydrozoline resulted in a visibly weaker inflammatory response. CONCLUSION: Retraction liquids insertion led to an acute inflammatory response of gingival tissue which in time assumed a chronic character. The inflammatory response to the administered tetrahydrozoline was significantly lower with complete reparation of gingival tissue. Taking this fact into account it is recommended as a potential retraction agent.
UNLABELLED: BACKROUND/AIM: Retraction agents for temporary vertical and lateral suppression of gingival tissue as well as bleeding control and fluid flow in the gingival sulcus are expected to have maximal efficiency without irreversible damage of local tissue and adverse systemic effects. The research started from the assumption that tetrahydrozoline is a biologically more acceptable means of gingival retraction than commercially available preparations. The aim of the study was to comparatively analyse the inflammatory effects of different retraction materials and tetrahydrozoline. METHODS: The effect of retraction liquid on the basis of aluminum chloride and epinephrine and tetrahydrozoline hydrochloride on gingival tissue of rabbits was investigated. The application time in the rabbit's gingival sulcus was 7 minutes. Tissue biopsy was performed after an hour, a day, and 7 and 30 days. Tissue preparations were analyzed under a microscope. RESULTS: The obtained results indicate a reversible damage of gingival tissues as a result of local application of aluminum chloride- and epinephrine-based retraction agents. Their use led to acute inflammatory response after an observation period of 1 and 7 days. After 30 days reparation of damaged tissue was observed. The use of tetrahydrozoline resulted in a visibly weaker inflammatory response. CONCLUSION: Retraction liquids insertion led to an acute inflammatory response of gingival tissue which in time assumed a chronic character. The inflammatory response to the administered tetrahydrozoline was significantly lower with complete reparation of gingival tissue. Taking this fact into account it is recommended as a potential retraction agent.
Authors: Marko Igic; Milena Kostic; Jelena Basic; Nebojsa Krunic; Ana Pejcic; Nikola Gligorijevic; Aleksandra Milic Lemic Journal: Med Princ Pract Date: 2020-04-02 Impact factor: 1.927