| Literature DB >> 24515466 |
Joyce Hui-Yuen1, Siva Koganti, Sumita Bhaduri-McIntosh.
Abstract
Infection of primary B lymphocytes with Epstein-Barr virus gives rise to growth-transformed and immortalized lymphoblastoid cell lines (LCL) in vitro. Among their many applications is the use of LCL to present antigens in a variety of immunologic assays and to generate human monoclonal antibodies. This chapter describes a method to generate LCL from donor peripheral blood with rapid immortalization and cryopreservation times.Entities:
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Year: 2014 PMID： 24515466 DOI： 10.1007/978-1-62703-992-5_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745