Literature DB >> 24515465

Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation.

James A Van Deventer1, Karl Dane Wittrup.   

Abstract

Antibodies play key roles as reagents, diagnostics, and therapeutics in numerous biological and biomedical research settings. Although many antibodies are commercially available, oftentimes, specific applications require the development of antibodies with customized properties. Yeast surface display is a robust, versatile, and quantitative method for generating these antibodies and is accessible to single-investigator laboratories. This protocol details the key aspects of yeast surface display library construction and screening.

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Year:  2014        PMID： 24515465     DOI： 10.1007/978-1-62703-992-5_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  26 in total

1.  Engineering a potent receptor superagonist or antagonist from a novel IL-6 family cytokine ligand.

Authors:  Jun W Kim; Cesar P Marquez; R Andres Parra Sperberg; Jiaxiang Wu; Won G Bae; Po-Ssu Huang; E Alejandro Sweet-Cordero; Jennifer R Cochran
Journal:  Proc Natl Acad Sci U S A       Date:  2020-06-10       Impact factor: 11.205

2.  Reduction of Nonspecificity Motifs in Synthetic Antibody Libraries.

Authors:  Ryan L Kelly; Doris Le; Jessie Zhao; K Dane Wittrup
Journal:  J Mol Biol       Date:  2017-11-26       Impact factor: 5.469

3.  Combining Phage and Yeast Cell Surface Antibody Display to Identify Novel Cell Type-Selective Internalizing Human Monoclonal Antibodies.

Authors:  Scott Bidlingmaier; Yang Su; Bin Liu
Journal:  Methods Mol Biol       Date:  2015

4.  A Robust and Quantitative Reporter System To Evaluate Noncanonical Amino Acid Incorporation in Yeast.

Authors:  Jessica T Stieglitz; Haixing P Kehoe; Ming Lei; James A Van Deventer
Journal:  ACS Synth Biol       Date:  2018-09-04       Impact factor: 5.110

5.  A platform for constructing, evaluating, and screening bioconjugates on the yeast surface.

Authors:  James A Van Deventer; Doris N Le; Jessie Zhao; Haixing P Kehoe; Ryan L Kelly
Journal:  Protein Eng Des Sel       Date:  2016-11-01       Impact factor: 1.650

6.  Deep Mutational Scans as a Guide to Engineering High Affinity T Cell Receptor Interactions with Peptide-bound Major Histocompatibility Complex.

Authors:  Daniel T Harris; Ningyan Wang; Timothy P Riley; Scott D Anderson; Nishant K Singh; Erik Procko; Brian M Baker; David M Kranz
Journal:  J Biol Chem       Date:  2016-09-28       Impact factor: 5.157

7.  Incorporating, Quantifying, and Leveraging Noncanonical Amino Acids in Yeast.

Authors:  Jessica T Stieglitz; James A Van Deventer
Journal:  Methods Mol Biol       Date:  2022

8.  Isolation of HCV Neutralizing Antibodies by Yeast Display.

Authors:  Zhen-Yong Keck; Yong Wang; Patrick Lau; Steven K H Foung
Journal:  Methods Mol Biol       Date:  2019

9.  Antitumor activity of an engineered decoy receptor targeting CLCF1-CNTFR signaling in lung adenocarcinoma.

Authors:  Jun W Kim; Cesar P Marquez; Kaja Kostyrko; Amanda L Koehne; Kieren Marini; David R Simpson; Alex G Lee; Stanley G Leung; Leanne C Sayles; Joseph Shrager; Irene Ferrer; Luis Paz-Ares; Melanie Hayden Gephart; Silvestre Vicent; Jennifer R Cochran; E Alejandro Sweet-Cordero
Journal:  Nat Med       Date:  2019-11-07       Impact factor: 53.440

10.  Chemical Diversification of Simple Synthetic Antibodies.

Authors:  Mariha Islam; Haixing P Kehoe; Jacob B Lissoos; Manjie Huang; Christopher E Ghadban; Greg Berumen Sánchez; Hanan Z Lane; James A Van Deventer
Journal:  ACS Chem Biol       Date:  2021-01-22       Impact factor: 5.100
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