Harnessing G-tetrad scaffolds within G-quadruplex forming aptamers for fluorescence detection strategies.

G-tetrads are essential structural components required for the formation of G-quadruplexes. Replacement of G nucleobases within G-tetrads with fluorescent 8-aryl-dG residues provides diagnostic handles that are universally applicable to antiparallel G-quadruplex aptamers, as they can stabilize quadruplex folding and maintain aptamer function.