Literature DB >> 2451325

Silver staining of the nucleolar organizer regions (NORs) in semithin Lowicryl sections.

F J Moreno1, A Villamarin, G Garcia-Herdugo, J L Lopez-Campos.   

Abstract

The one-step silver technique was applied to semithin Lowicryl sections of root meristem cells of Allium cepa and a human tumor cell line (TG cells). In vegetal cells, after 5 min of staining reaction, the Ag-NOR proteins formed ring-shaped structures peripherally within the nucleolus. In animal cells silver granules were distributed over the entire nucleolus. The specificity of the staining reaction was increased by incubation of the sections in NH4Cl and Schiff's reagent prior to Ag-NOR silver staining.

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Year:  1988        PMID: 2451325     DOI: 10.3109/10520298809107156

Source DB:  PubMed          Journal:  Stain Technol        ISSN: 0038-9153


  4 in total

Review 1.  Molecular biology and respiratory disease. 3. Evaluation of nucleolar organiser regions in pulmonary pathology.

Authors:  M J Egan; J Crocker
Journal:  Thorax       Date:  1990-03       Impact factor: 9.139

2.  A new version of the Ag-NOR technique. A combination with DAPI staining.

Authors:  S M Biliński; B Bilińska
Journal:  Histochem J       Date:  1996-09

3.  Microwave irradiation improvements in the silver staining of the nucleolar organizer (Ag-NOR) technique.

Authors:  F J Medina; A Cerdido; R Marco
Journal:  Histochem Cell Biol       Date:  1995-06       Impact factor: 4.304

Review 4.  Nucleolar organiser regions in pathology.

Authors:  M J Egan; J Crocker
Journal:  Br J Cancer       Date:  1992-01       Impact factor: 7.640

  4 in total

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