Sinibacillus soli gen. nov., sp. nov., a moderately thermotolerant member of the family Bacillaceae.

Two Gram-staining-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains GD05T and GD051, were isolated from a tropical forest soil and a hot spring sediment, respectively. Cells of both strains were facultatively anaerobic, catalase- and oxidase-positive, and could grow optimally at 50 °C, pH 8.0 and with 1 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that these two isolates belonged to the family Bacillaceae, but did not show sequence similarities of more than 95% to members of other related genera. The G+C content of the genomic DNA was 43.7-44.1 mol%. The major cellular fatty acids were anteiso-C15:0, iso-C15:0, iso-C16:0 and anteiso-C17:0. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major menaquinone was MK-7. The peptidoglycan type was A1γ (meso-diaminopimelic acid direct). On the basis of this polyphasic taxonomic analysis, the novel strains represent a novel species of a new genus in the family Bacillaceae, order Bacillales, for which the name Sinibacillus soli gen. nov., sp. nov. is proposed. The type strain is GD05T (=CCTCC AB 2013105T=KCTC 33117T).