Literature DB >> 24510881

Assays to examine endothelial cell migration, tube formation, and gene expression profiles.

Shuzhen Guo1, Josephine Lok, Yi Liu, Kazuhide Hayakawa, Wendy Leung, Changhong Xing, Xunming Ji, Eng H Lo.   

Abstract

Common methods for studying angiogenesis in vitro include the tube formation assay, the migration assay, and the study of the endothelial genome. The formation of capillary-like tubes in vitro on basement membrane matrix mimics many steps of the angiogenesis process in vivo and is used widely as a screening test for angiogenic or antiangiogenic factors. Other assays related to the study of angiogenesis include the cell migration assay, the study of gene expression changes during the process of angiogenesis, and the study of endothelial-derived microparticles. Protocols for these procedures will be described here.

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Year:  2014        PMID： 24510881      PMCID： PMC4035906          DOI： 10.1007/978-1-4939-0320-7_32

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

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Authors:  Hyun Kyung Kim; Kyung Soon Song; Jun-Ho Chung; Kyoung Rhan Lee; Se-Na Lee
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Review 4.  The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art.

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5.  In vitro three dimensional collagen matrix models of endothelial lumen formation during vasculogenesis and angiogenesis.

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Journal:  Methods Enzymol       Date:  2008       Impact factor: 1.600

6.  Tumor-promoting phorbol esters induce angiogenesis in vitro.

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7.  Neurovascular effects of CD47 signaling: promotion of cell death, inflammation, and suppression of angiogenesis in brain endothelial cells in vitro.

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9.  Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures.

Authors:  Y Kubota; H K Kleinman; G R Martin; T J Lawley
Journal:  J Cell Biol       Date:  1988-10       Impact factor: 10.539

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  11 in total

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5.  Associations between fetal size, sex and placental angiogenesis in the pig.

Authors:  Claire Stenhouse; Charis O Hogg; Cheryl J Ashworth
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6.  High glucose conditioned neonatal astrocytes results in impaired mitogenic activity in cerebral microvessel endothelial cells in co-culture.

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7.  Nox2 contributes to age-related oxidative damage to neurons and the cerebral vasculature.

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8.  CD73+ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling.

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9.  Resolvin D1 suppresses pannus formation via decreasing connective tissue growth factor caused by upregulation of miRNA-146a-5p in rheumatoid arthritis.

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10.  PPARβ/δ selectively regulates phenotypic features of age-related macular degeneration.

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