Literature DB >> 24510437

Library generation by gene shuffling.

Adam J Meyer1, Jared W Ellefson, Andrew D Ellington.   

Abstract

This unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer-less PCR. The resulting chimeric genes can then be screened or selected for a desired function.
Copyright © 2014 John Wiley & Sons, Inc.

Entities:  

Keywords:  PCR; directed evolution; recombination

Mesh：

Substances：

Year:  2014        PMID： 24510437      PMCID： PMC3983707          DOI： 10.1002/0471142727.mb1512s105

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  14 in total

1.  Analysis of shuffled gene libraries.

Authors:  John M Joern; Peter Meinhold; Frances H Arnold
Journal:  J Mol Biol       Date:  2002-02-22       Impact factor: 5.469

2.  Combinatorial recombination of gene fragments to construct a library of chimeras.

Authors:  Mary F Farrow; Frances H Arnold
Journal:  Curr Protoc Protein Sci       Date:  2010-08

3.  Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function.

Authors:  K Miyazaki; F H Arnold
Journal:  J Mol Evol       Date:  1999-12       Impact factor: 2.395

4.  Sequence-independent construction of ordered combinatorial libraries with predefined crossover points.

Authors:  Laetitia Jézéquel; Jacqueline Loeper; Denis Pompon
Journal:  Biotechniques       Date:  2008-11       Impact factor: 1.993

5.  Intense neutral drifts yield robust and evolvable consensus proteins.

Authors:  Shimon Bershtein; Korina Goldin; Dan S Tawfik
Journal:  J Mol Biol       Date:  2008-04-16       Impact factor: 5.469

6.  Tuning biphenyl dioxygenase for extended substrate specificity.

Authors:  F Brühlmann; W Chen
Journal:  Biotechnol Bioeng       Date:  1999-06-05       Impact factor: 4.530

7.  Random recombination of antibody single chain Fv sequences after fragmentation with DNaseI in the presence of Mn2+.

Authors:  I A Lorimer; I Pastan
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

8.  Directed evolution of adeno-associated virus yields enhanced gene delivery vectors.

Authors:  Narendra Maheshri; James T Koerber; Brian K Kaspar; David V Schaffer
Journal:  Nat Biotechnol       Date:  2006-01-22       Impact factor: 54.908

9.  Evolution of a human immunodeficiency virus type 1 variant with enhanced replication in pig-tailed macaque cells by DNA shuffling.

Authors:  Katja Pekrun; Riri Shibata; Tatsuhiko Igarashi; Margaret Reed; Liana Sheppard; Philip A Patten; Willem P C Stemmer; Malcolm A Martin; Nay-Wei Soong
Journal:  J Virol       Date:  2002-03       Impact factor: 5.103

10.  Optimization of DNA shuffling for high fidelity recombination.

Authors:  H Zhao; F H Arnold
Journal:  Nucleic Acids Res       Date:  1997-03-15       Impact factor: 16.971
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  5 in total

Review 1.  The Toolbox for Modified Aptamers.

Authors:  Sergey A Lapa; Alexander V Chudinov; Edward N Timofeev
Journal:  Mol Biotechnol       Date:  2016-02       Impact factor: 2.695

2.  Highly protective antimalarial antibodies via precision library generation and yeast display screening.

Authors:  Bailey B Banach; Prabhanshu Tripathi; Lais Da Silva Pereira; Jason Gorman; Thuy Duong Nguyen; Marlon Dillon; Ahmed S Fahad; Patience K Kiyuka; Bharat Madan; Jacy R Wolfe; Brian Bonilla; Barbara Flynn; Joseph R Francica; Nicholas K Hurlburt; Neville K Kisalu; Tracy Liu; Li Ou; Reda Rawi; Arne Schön; Chen-Hsiang Shen; I-Ting Teng; Baoshan Zhang; Marie Pancera; Azza H Idris; Robert A Seder; Peter D Kwong; Brandon J DeKosky
Journal:  J Exp Med       Date:  2022-06-23       Impact factor: 17.579

3.  Directed Evolution of Phi Class Glutathione Transferases Involved in Multiple-Herbicide Resistance of Grass Weeds and Crops.

Authors:  Elisavet Ioannou; Anastassios C Papageorgiou; Nikolaos E Labrou
Journal:  Int J Mol Sci       Date:  2022-07-05       Impact factor: 6.208

4.  Laboratory evolution of a sortase enzyme that modifies amyloid-β protein.

Authors:  Christopher J Podracky; Chihui An; Alexandra DeSousa; Brent M Dorr; Dominic M Walsh; David R Liu
Journal:  Nat Chem Biol       Date:  2021-01-11       Impact factor: 15.040

5.  Characterizing chemical signaling between engineered "microbial sentinels" in porous microplates.

Authors:  Christopher A Vaiana; Hyungseok Kim; Jonathan Cottet; Keiko Oai; Zhifei Ge; Kameron Conforti; Andrew M King; Adam J Meyer; Haorong Chen; Christopher A Voigt; Cullen R Buie
Journal:  Mol Syst Biol       Date:  2022-03       Impact factor: 11.429
  5 in total

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