Literature DB >> 24510259

Mapping homing endonuclease cleavage sites using in vitro generated protein.

Richard P Bonocora1, Marlene Belfort.   

Abstract

Mapping the precise position of endonucleolytic cleavage sites is a fundamental experimental technique used to describe the function of a homing endonuclease. However, these proteins are often recalcitrant to cloning and over-expression in biological systems because of toxicity induced by spurious DNA cleavage events. In this chapter we outline the steps to successfully express a homing endonuclease in vitro and use this product in nucleotide-resolution cleavage assays.

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Year:  2014        PMID： 24510259      PMCID： PMC4435548          DOI： 10.1007/978-1-62703-968-0_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  27 in total

1.  Identification of a family of bacteriophage T4 genes encoding proteins similar to those present in group I introns of fungi and phage.

Authors:  M Sharma; R L Ellis; D M Hinton
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

2.  I-TevI, the endonuclease encoded by the mobile td intron, recognizes binding and cleavage domains on its DNA target.

Authors:  D Bell-Pedersen; S M Quirk; M Bryk; M Belfort
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-01       Impact factor: 11.205

3.  Intron mobility in phage T4 is dependent upon a distinctive class of endonucleases and independent of DNA sequences encoding the intron core: mechanistic and evolutionary implications.

Authors:  D Bell-Pedersen; S Quirk; J Clyman; M Belfort
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

4.  A site-specific endonuclease and co-conversion of flanking exons associated with the mobile td intron of phage T4.

Authors:  D Bell-Pedersen; S M Quirk; M Aubrey; M Belfort
Journal:  Gene       Date:  1989-10-15       Impact factor: 3.688

5.  Intronless homing: site-specific endonuclease SegF of bacteriophage T4 mediates localized marker exclusion analogous to homing endonucleases of group I introns.

Authors:  Archana Belle; Markus Landthaler; David A Shub
Journal:  Genes Dev       Date:  2002-02-01       Impact factor: 11.361

6.  Highly repressible expression system for cloning genes that specify potentially toxic proteins.

Authors:  C D O'Connor; K N Timmis
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

7.  T7 lysozyme inhibits transcription by T7 RNA polymerase.

Authors:  B A Moffatt; F W Studier
Journal:  Cell       Date:  1987-04-24       Impact factor: 41.582

8.  Determinant of cistron specificity in bacterial ribosomes.

Authors:  J Shine; L Dalgarno
Journal:  Nature       Date:  1975-03-06       Impact factor: 49.962

9.  The chemical synthesis of a gene coding for bovine pancreatic DNase I and its cloning and expression in Escherichia coli.

Authors:  A F Worrall; B A Connolly
Journal:  J Biol Chem       Date:  1990-12-15       Impact factor: 5.157

10.  Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system.

Authors:  F W Studier
Journal:  J Mol Biol       Date:  1991-05-05       Impact factor: 5.469
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  1 in total

1.  The Characteristics and Genome Analysis of vB_AviM_AVP, the First Phage Infecting Aerococcus viridans.

Authors:  Hengyu Xi; Jiaxin Dai; Yigang Tong; Mengjun Cheng; Feiyang Zhao; Hang Fan; Xinwei Li; Ruopeng Cai; Yalu Ji; Changjiang Sun; Xin Feng; Liancheng Lei; Sadeeq Ur Rahman; Wenyu Han; Jingmin Gu
Journal:  Viruses       Date:  2019-01-26       Impact factor: 5.048
  1 in total

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