Literature DB >> 24508590

A semi-automated method for purification of milligram quantities of proteins on the QIAcube.

J McGraw1, V K Tatipelli1, O Feyijinmi1, M C Traore1, P Eangoor1, S Lane1, E J Stollar2.   

Abstract

A growing number of studies require the purification of multiple proteins simultaneously and the development of simple economical high-throughput purification methods is essential. We have tested the purification of two related proteins in a variety of conditions to benchmark the semi-automated affinity chromatography method for the QIAcube that we have developed. We find that this new QIAcube method can successfully purify milligram quantities of proteins with minimal user involvement and performs as well as methods based on gravity. The method could easily be adapted to other chromatography resins and should prove to be a versatile method for optimizing protein expression or purification conditions for multiple proteins while obtaining sufficient amounts for subsequent biochemical analyses.
Copyright © 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  High-throughput automation; Protein expression; Protein purification; QIAcube; SH3 domain

Mesh：

Substances：

Year:  2014        PMID： 24508590      PMCID： PMC4287359          DOI： 10.1016/j.pep.2014.01.014

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


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