Pai-Fu Wang1, Allen W Chiu2, Yueh-Min Lin3, Ching-Yuang Lin4, Hung-Jen Shih5. 1. Division of Urology, Department of Surgery, Changhua Christian Hospital, Taiwan. 2. School of Medicine, National Yang-Ming University, Taiwan. 3. Department of Pathology, Changhua Christian Hospital, Taiwan. 4. Division of Pediatric Nephrology, Children's Medical Center, China Medical University Hospital, Taiwan. 5. Division of Urology, Department of Surgery, Changhua Christian Hospital, Taiwan. Electronic address: jasta1206@gmail.com.
Abstract
BACKGROUND: To evaluate the clinical efficacy and histochemical impact of a new technique of renal repair using a fibrin sealant and Dexon mesh in rats. METHODS: Ten groups of Sprague-Dawley (SD) rats underwent a bilateral partial nephrectomy 30, 21, 14, 7 to 1 days before sacrifice. Renal repair was accomplished by suturing on one side and using fibrin sealant and Dexon mesh on the opposite side. The time for renal reconstruction was recorded for each approach and compared. In addition to histological evaluations, the isolated renal tissue studies included immunohistochemical analysis, and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: In comparison with suturing, renal repair using fibrin sealant and Dexon mesh was much faster. We demonstrated a significant attenuation of the initial inflammatory response in the fibrin-Dexon group. The specific alterations in transforming growth factor-β1 (Tgf-β1) mRNA expression were significantly lower in the fibrin-Dexon group. CONCLUSIONS: The fibrin sealant and Dexon mesh significantly simplified the procedure by reducing the time of renal reconstruction. This approach can diminish the fibrotic reaction and offers a response for renal repair similar to the suturing technique.
BACKGROUND: To evaluate the clinical efficacy and histochemical impact of a new technique of renal repair using a fibrin sealant and Dexon mesh in rats. METHODS: Ten groups of Sprague-Dawley (SD) rats underwent a bilateral partial nephrectomy 30, 21, 14, 7 to 1 days before sacrifice. Renal repair was accomplished by suturing on one side and using fibrin sealant and Dexon mesh on the opposite side. The time for renal reconstruction was recorded for each approach and compared. In addition to histological evaluations, the isolated renal tissue studies included immunohistochemical analysis, and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: In comparison with suturing, renal repair using fibrin sealant and Dexon mesh was much faster. We demonstrated a significant attenuation of the initial inflammatory response in the fibrin-Dexon group. The specific alterations in transforming growth factor-β1 (Tgf-β1) mRNA expression were significantly lower in the fibrin-Dexon group. CONCLUSIONS: The fibrin sealant and Dexon mesh significantly simplified the procedure by reducing the time of renal reconstruction. This approach can diminish the fibrotic reaction and offers a response for renal repair similar to the suturing technique.
Authors: Wei-Ling Luo; Jun Zhang; Xuan Qiu; Li-Juan Chen; Jie Fu; Peng-Yue Hu; Xin Li; Ren-Jie Hu; Yun-Ze Long Journal: Nanoscale Res Lett Date: 2018-09-10 Impact factor: 4.703