Zhen-Chang Wang1, Shan Yang2, Jing-Jing Huang3, Song-Lin Chen3, Quan-Qiang Li2, Yuan Li3. 1. Liver Disease Center, First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Naning 530023, China. Electronic address: wangzhenchang924@163.com. 2. Guangxi University of Traditional Chinese Medicine, Nanning 530007, China. 3. Liver Disease Center, First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Naning 530023, China.
Abstract
OBJECTIVE: To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosis rats. METHODS: A total of 48 adult female SD rats were randomly divided into three groups, normal control group (n=10), observation group (n=19) with liver fibrosis model rats injected with BMSCs cells; model group (n=19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected. RESULTS: Type III procollagen, IV collagen, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P<0.05). The content and expression of TGF-β1 in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P<0.05). Compared with model group, Smad3, Smad4 mRNA and protein expression of observation group were significantly reduced, and Smad7 mRNA expression were significantly increased (P<0.05). CONCLUSIONS: BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.
OBJECTIVE: To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosisrats. METHODS: A total of 48 adult female SD rats were randomly divided into three groups, normal control group (n=10), observation group (n=19) with liver fibrosis model rats injected with BMSCs cells; model group (n=19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected. RESULTS: Type III procollagen, IV collagen, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P<0.05). The content and expression of TGF-β1 in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P<0.05). Compared with model group, Smad3, Smad4 mRNA and protein expression of observation group were significantly reduced, and Smad7 mRNA expression were significantly increased (P<0.05). CONCLUSIONS: BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.
Authors: Pu Huang; Yan Zhou; Xiao-Hong Li; Yun-Na Zhang; Hai-Peng Cheng; Jia-Feng Fu; Wei Liu; Shaojie Yue; Zi-Qiang Luo Journal: Ann Transl Med Date: 2022-06