Jun-Jie Chen1, Jie-Feng Huang1, Wen-Xi Du1, Pei-Jian Tong2. 1. Department of Orthopedics, First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China. 2. Department of Orthopedics, First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China. Electronic address: Tongpeijian@163.com.
Abstract
OBJECTIVE: To investigate the expression and significance of MMP-3 in synovium of knee joint at different stage in osteoarthritis (OA) patients. METHODS: Knee synovial tissue were collected in 90 OA patients (the OA group). Patients in the OA group was divided into 3 subgroups: grade I subgroup (n=30), grade II subgroup (n=30), grade III; subgroup (n=30). Thirty patients served as control group. Immunohistochemical assay was used to detect the expression of MMP-3 protein in the knee synovial tissue. RESULTS: MMP-3 protein was detected in all knee synovial tissue. The expression of MMP-3 protein in the OA group was significantly higher that in the normal synovium (P<0.05), and the MMP-3 protein was mainly located in the cytoplasm. There was significant difference in the expression of MMP-3 protein between the grade I subgroup and the grade II, grade III subgroups (all P<0.05). The expression of MMP-3 protein was positively related to the severity of OA (r=0.912, P<0.05). CONCLUSIONS: The expression of MMP-3 protein are closely related to pathogenic mechanism of OA. It may be an important indicator of early diagnosis and the activity of the disease of osteoarthritis.
OBJECTIVE: To investigate the expression and significance of MMP-3 in synovium of knee joint at different stage in osteoarthritis (OA) patients. METHODS: Knee synovial tissue were collected in 90 OA patients (the OA group). Patients in the OA group was divided into 3 subgroups: grade I subgroup (n=30), grade II subgroup (n=30), grade III; subgroup (n=30). Thirty patients served as control group. Immunohistochemical assay was used to detect the expression of MMP-3 protein in the knee synovial tissue. RESULTS:MMP-3 protein was detected in all knee synovial tissue. The expression of MMP-3 protein in the OA group was significantly higher that in the normal synovium (P<0.05), and the MMP-3 protein was mainly located in the cytoplasm. There was significant difference in the expression of MMP-3 protein between the grade I subgroup and the grade II, grade III subgroups (all P<0.05). The expression of MMP-3 protein was positively related to the severity of OA (r=0.912, P<0.05). CONCLUSIONS: The expression of MMP-3 protein are closely related to pathogenic mechanism of OA. It may be an important indicator of early diagnosis and the activity of the disease of osteoarthritis.
Authors: Kristen I Barton; Bryan J Heard; May Chung; Johnathan L Sevick; C Ryan Martin; Yamini Achari; Cyril B Frank; Nigel G Shrive; David A Hart Journal: Inflamm Res Date: 2016-11-16 Impact factor: 4.575
Authors: Sagar Singh; Sonia Kartha; Ben A Bulka; Nicholas S Stiansen; Beth A Winkelstein Journal: Clin Biomech (Bristol, Avon) Date: 2018-01-31 Impact factor: 2.063
Authors: L A Deveza; V B Kraus; J E Collins; A Guermazi; F W Roemer; M C Nevitt; D J Hunter Journal: Scand J Rheumatol Date: 2017-09-20 Impact factor: 3.641
Authors: Christoph Meinert; Karsten Schrobback; Peter A Levett; Cameron Lutton; Robert L Sah; Travis J Klein Journal: Acta Biomater Date: 2016-10-08 Impact factor: 8.947
Authors: Eric A Lewallen; Christopher G Salib; William H Trousdale; Charlotte E Berry; Gabrielle M Hanssen; Joseph X Robin; Meagan E Tibbo; Anthony Viste; Nicolas Reina; Mark E Morrey; Joaquin Sanchez-Sotelo; Arlen D Hanssen; Daniel J Berry; Andre J van Wijnen; Matthew P Abdel Journal: Genomics Date: 2017-11-22 Impact factor: 5.736