Rui Wang1, Chang-zheng Zhu2, Ping Qiao3, Jian Liu3, Qiang Zhao3, Kui-jie Wang2, Ting-bao Zhao4. 1. Department of Spinal Cord Repairing, Jinan Military General Hospital, Jinan 250031, China. 2. Department of Neurology, People's Hospital of Zhangqiu, Zhangqiu 250200, China. 3. Department of Orthopedics, People's Hospital of Zhangqiu, Zhangqiu 250200, China. 4. Department of Spinal Cord Repairing, Jinan Military General Hospital, Jinan 250031, China. Electronic address: woaifei371328@163.com.
Abstract
OBJECTIVE: To evaluate of the curative effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on rat acute radiation pneumonitis. METHODS: Fourty rats were randomly divided into control group, radiation group, stem cell prevention group, stem cell treatment group and prednisone treatment group. All rats except those in the control group were radiated with X ray to establish the acute radiation pneumonitis damage model. The hUC-MSCs cultured in vitro was administrated to the rats of the prevention group via tail vein (1×10(6) cells/kg BW) 24 h before the radiation, while the same administration was performed in the rats of the treatment group 24 h after the radiation. After 24 h post the radiation, the rats in the radiation group were given 0.4 mL physiological saline, and those in the prednisone group were given 1 mg/kg prednisone. All rats were observed and executed 72 h after the radiation to detect lung histological changes. RESULTS: After the administration of hUC-MSCs, the survival status of the rats in the prevention group and treatment group was obviously better than that in the control group. As shown by the histological staining, the morphology, proliferation activity and bronchial state of lung tissues were better in the prevention group and treatment group than in the control group. CONCLUSIONS: The hUC-MSCs have definite therapeutic effects on acute radiation pneumonitis in rats.
OBJECTIVE: To evaluate of the curative effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on rat acute radiation pneumonitis. METHODS: Fourty rats were randomly divided into control group, radiation group, stem cell prevention group, stem cell treatment group and prednisone treatment group. All rats except those in the control group were radiated with X ray to establish the acute radiation pneumonitis damage model. The hUC-MSCs cultured in vitro was administrated to the rats of the prevention group via tail vein (1×10(6) cells/kg BW) 24 h before the radiation, while the same administration was performed in the rats of the treatment group 24 h after the radiation. After 24 h post the radiation, the rats in the radiation group were given 0.4 mL physiological saline, and those in the prednisone group were given 1 mg/kg prednisone. All rats were observed and executed 72 h after the radiation to detect lung histological changes. RESULTS: After the administration of hUC-MSCs, the survival status of the rats in the prevention group and treatment group was obviously better than that in the control group. As shown by the histological staining, the morphology, proliferation activity and bronchial state of lung tissues were better in the prevention group and treatment group than in the control group. CONCLUSIONS: The hUC-MSCs have definite therapeutic effects on acute radiation pneumonitis in rats.
Authors: Vito Mahendra Ekasaputra; Agung Putra; Adi Muradi Muhar; Jherin Varessa; Rafeni Bunga Cikita; Satya A P Wijaya; Mohammad Ariq Nazar Journal: Med Arch Date: 2020-10