Physicochemical characterization of amphiphilic nanoparticles based on the novel starch-deoxycholic acid conjugates and self-aggregates.

Novel amphiphilic polymers (starch-deoxycholic acid, St-DCA) were firstly synthesized on the basis of starch (St) as a hydrophilic segment and deoxycholic acid (DCA) as a hydrophobic segment. Hydrophobically modified starch contained 5.4-8.9 deoxycholic acid groups per 100 anhydroglucose units of starch. Self-aggregates of St-DCA conjugates were formed in the PBS media. Physicochemical characterizations of St-DCA conjugates were investigated. The mean sizes of self-aggregates decreased with the degree of substitution (DS) and pH increasing. Zeta potential indicated that nanoparticles were covered with negatively charged starch shells from -5.4 to -23 mV. TEM images demonstrated that nanoparticles were of spherical shape. The critical aggregation concentrations (cac) were dependent on the DS and pH in the range of 0.0185-0.0441 mg/mL. Thus, the study suggested that self-aggregated nanoparticles of St-DCA conjugates could have good pH-responsive and potential application in pharmaceutical and biomedical fields as the delivery of anti-tumor drugs.